Overview

  • Product name

    Anti-NHLRC1/Malin antibody [N85/18]
    See all NHLRC1/Malin primary antibodies
  • Description

    Mouse monoclonal [N85/18] to NHLRC1/Malin
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human NHLRC1/Malin aa 2-125. N-terminal encompassing RING domain.
    Database link: Q6VVB1

  • Positive control

    • Lysate from COS cells transiently transfected with NHLRC1/Malin.
  • General notes

    The clone number has been updated from S85-18 to N85/18, both clone numbers name the same antibody clone.

     

     This product was previously labelled as NHLRC1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab186261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Function

    E3 ubiquitin-protein ligase. Together with the phosphatase EPM2A/laforin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. In complex with EPM2A/laforin and HSP70, suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Ubiquitinates the glycogen-targeting protein phosphatase subunits PPP1R3C/PTG and PPP1R3D in a laforin-dependent manner and targets them for proteasome-dependent degradation, thus decreasing glycogen accumulation. Polyubiquitinates EPM2A/laforin and ubiquitinates AGL and targets them for proteasome-dependent degradation. Also promotes proteasome-independent protein degradation through the macroautophagy pathway.
  • Tissue specificity

    Expressed in brain, cerebellum, spinal cord, medulla, heart, liver, skeletal muscle and pancreas.
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Epilepsy, progressive myoclonic 2
  • Sequence similarities

    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING domain is essential for ubiquitin E3 ligase activity.
  • Cellular localization

    Endoplasmic reticulum. Nucleus. Localizes at the endoplasmic reticulum and, to a lesser extent, in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA204B7.2 antibody
    • E3 ubiquitin-protein ligase NHLRC1 antibody
    • EPM2A antibody
    • EPM2B antibody
    • EPM2B gene antibody
    • Malin antibody
    • MGC119262 antibody
    • MGC119264 antibody
    • MGC119265 antibody
    • NHL repeat containing 1 antibody
    • NHL repeat containing E3 ubiquitin protein ligase 1 antibody
    • NHL repeat containing protein 1 antibody
    • NHL repeat-containing protein 1 antibody
    • NHL repeat-containing protein 1 gene antibody
    • NHLC1_HUMAN antibody
    • NHLRC 1 antibody
    • Nhlrc1 antibody
    see all

Images

  • ICC/IF analysis of 4% paraformaldehyde-fixed human SK-N-BE (Neuroblastoma cell line) stained for NHLRC1/Malin (green) using ab186261 at 1/100 dilution. Goat Anti-Mouse ATTO 488 was used as the secondary at 1/100 dilution. Counterstained with Phalloidin Texas Red F-Actin stain (1/1000) , and DAPI, Blue (nuclear counterstain) at 1/5000 dilution. Staining in cytoplasm, Endoplasmic Reticulum is observed. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Malin Antibody. (D) Composite.

  • Anti-SLITRK2 antibody (ab186361) at 1/200 dilution + Monkey COS cells transfected with flag-tagged Malin at 15 µg

    Secondary
    Goat Anti-Mouse IgG: HRP at 1/1000 dilution

    Predicted band size: 42 kDa

References

ab186261 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab186261.
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