Key features and details
- Mouse polyclonal to NHLRC2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NHLRC2 antibody
See all NHLRC2 primary antibodies
DescriptionMouse polyclonal to NHLRC2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length Human protein (AAH32598)
- Human pancreas lysate and NHLRC2 transfected 293T cell lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 79 kDa.|
Sequence similaritiesContains 6 NHL repeats.
Contains 1 thioredoxin domain.
- Information by UniProt
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Anti-NHLRC2 antibody (ab88725) at 1/500 dilution + Human pancreas lysate at 50 µg
Predicted band size: 79 kDa
Observed band size: 45,79 kDa why is the actual band size different from the predicted?
All lanes : Anti-NHLRC2 antibody (ab88725) at 1/500 dilution
Lane 1 : NHLRC2 transfected 293T cell lysate
Lane 2 : non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 79 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
ab88725 has been referenced in 1 publication.
- Nishi K et al. ROS-induced cleavage of NHLRC2 by caspase-8 leads to apoptotic cell death in the HCT116 human colon cancer cell line. Cell Death Dis 8:3218 (2017). PubMed: 29242562