Overview

  • Product name

  • Description

    Mouse polyclonal to NHLRC2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length Human protein (AAH32598)

  • Positive control

    • Human pancreas lysate and NHLRC2 transfected 293T cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 79 kDa.

Target

  • Sequence similarities

    Contains 6 NHL repeats.
    Contains 1 thioredoxin domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1200003G01Rik antibody
    • DKFZp779F115 antibody
    • FLJ20147 antibody
    • FLJ25621 antibody
    • FLJ33312 antibody
    • MGC45492 antibody
    • NHL repeat containing 2 antibody
    • NHL repeat-containing protein 2 antibody
    • NHLC2_HUMAN antibody
    • NHLRC2 antibody
    • Novel NHL repeat domain containing protein antibody
    • OTTHUMP00000020518 antibody
    see all

Images

  • Anti-NHLRC2 antibody (ab88725) at 1/500 dilution + Human pancreas lysate at 50 µg

    Predicted band size: 79 kDa
    Observed band size: 45,79 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-NHLRC2 antibody (ab88725) at 1/500 dilution

    Lane 1 : NHLRC2 transfected 293T cell lysate
    Lane 2 : non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 79 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Nishi K  et al. ROS-induced cleavage of NHLRC2 by caspase-8 leads to apoptotic cell death in the HCT116 human colon cancer cell line. Cell Death Dis 8:3218 (2017). Read more (PubMed: 29242562) »
See 1 Publication for this product

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