Overview

  • Product name

    Anti-NHP2 antibody [EPR12404]
    See all NHP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12404] to NHP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NHP2 aa 1-100. The exact sequence is proprietary.
    Database link: Q9NX24

  • Positive control

    • K562, Jurkat, Caco 2 and SH-SY5Y lysates; Human ovarian carcinoma tissue; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Target

  • Function

    Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
  • Tissue specificity

    Expressed in brain, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Also expressed at lower levels in the liver.
  • Involvement in disease

    Dyskeratosis congenita, autosomal recessive, 2
  • Sequence similarities

    Belongs to the ribosomal protein L7Ae family.
  • Developmental stage

    Transcript peaks at G1/S transition.
  • Cellular localization

    Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKCB2 antibody
    • FLJ20479 antibody
    • H/ACA ribonucleoprotein complex subunit 2 antibody
    • NHP2 antibody
    • NHP2 like protein antibody
    • NHP2 ribonucleoprotein antibody
    • NHP2 ribonucleoprotein homolog (yeast) 1 antibody
    • NHP2 ribonucleoprotein homolog antibody
    • NHP2, S. cerevisiae, homolog of antibody
    • NHP2_HUMAN antibody
    • NHP2P antibody
    • NOLA2 antibody
    • Nucleolar protein family A member 2 (H/ACA small nucleolar RNPs) antibody
    • Nucleolar protein family A member 2 antibody
    • snoRNP protein NHP2 antibody
    see all

Images

  • All lanes : Anti-NHP2 antibody [EPR12404] (ab180498) at 1/2000 dilution

    Lane 1 : K562 lysate
    Lane 2 : Jurkat lysate
    Lane 3 : Caco 2 lysate
    Lane 4 : SH-SY5Y lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling NHP2 with ab180498 at 1/500 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG and counter stained with Hematoxylin.

  • Flow Cytometric analysis of K562 cells (2% paraformaldehyde-fixed) labeling NHP2 with ab180498 at 1/20 (red) compared to a Rabbit monoclonal IgG negative control antibody (green) followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.

References

This product has been referenced in:

  • Quan Wang J  et al. Discovery of new chromen-4-one derivatives as telomerase inhibitors through regulating expression of dyskerin. J Enzyme Inhib Med Chem 33:1199-1211 (2018). Read more (PubMed: 30132373) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (human embryonic stem cell, 293T, HeLa)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
50 µg
Specification
human embryonic stem cell, 293T, HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Jianlong Wang

Verified customer

Submitted Mar 19 2018

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