Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12404] to NHP2
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-NHP2 antibody [EPR12404]
See all NHP2 primary antibodies
DescriptionRabbit monoclonal [EPR12404] to NHP2
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NHP2 aa 1-100. The exact sequence is proprietary.
Database link: Q9NX24
- K562, Jurkat, Caco 2 and SH-SY5Y lysates; Human ovarian carcinoma tissue; K562 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).|
FunctionRequired for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Tissue specificityExpressed in brain, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Also expressed at lower levels in the liver.
Involvement in diseaseDyskeratosis congenita, autosomal recessive, 2
Sequence similaritiesBelongs to the ribosomal protein L7Ae family.
Developmental stageTranscript peaks at G1/S transition.
Cellular localizationNucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
- Information by UniProt
- DKCB2 antibody
- FLJ20479 antibody
- H/ACA ribonucleoprotein complex subunit 2 antibody
All lanes : Anti-NHP2 antibody [EPR12404] (ab180498) at 1/2000 dilution
Lane 1 : K562 lysate
Lane 2 : Jurkat lysate
Lane 3 : Caco 2 lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling NHP2 with ab180498 at 1/500 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG and counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow Cytometric analysis of K562 cells (2% paraformaldehyde-fixed) labeling NHP2 with ab180498 at 1/20 (red) compared to a Rabbit monoclonal IgG negative control antibody (green) followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.
ab180498 has been referenced in 1 publication.
- Quan Wang J et al. Discovery of new chromen-4-one derivatives as telomerase inhibitors through regulating expression of dyskerin. J Enzyme Inhib Med Chem 33:1199-1211 (2018). PubMed: 30132373