Overview

  • Product name

  • Description

    Rabbit polyclonal to NHP2L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human NHP2L1 aa 1-98.
    Database link: P55769

  • Positive control

    • WB: HeLa and HepG2 whole cell lysates ICC/IF: Hela cell IHC-P: Cal27 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab95958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 14 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • Relevance

    Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri snRNP. It binds to the 5' stem loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene.
  • Cellular localization

    Nucleus › nucleolus. Note: Concentrated in the dense fibrillar component of the nucleolus.
  • Database links

  • Alternative names

    • [U4/U6.U5] tri snRNP antibody
    • 15.5 kD RNA binding protein antibody
    • High mobility group like nuclear protein 2 homolog 1 antibody
    • hSNU13 antibody
    • NHP2 non histone chromosome protein 2 like 1 (S. cerevisiae) antibody
    • NHP2L 1 antibody
    • NHP2L1 antibody
    • NHPX antibody
    • OTK27 antibody
    • SNU13 antibody
    see all

Images

  • All lanes : Anti-NHP2L1 antibody (ab95958) at 1/1000 dilution

    Lane 1 : Hela whole cell lysate

    Lane 2 : HepG2 whole cell lysate


    Lysates/proteins at 30 µg per lane.

    Predicted band size: 14 kDa



    12% SDS PAGE
  • ab95958, at a 1/200 dilution, staining NHP2L1 in paraformaldehyde fixed HeLa cell by Immunofluorescence analysis.
  • ab95958, at a 1/100 dilution, staining NHP2L1 in paraffin embedded Cal27 xenograft by Immunohistochemistry.

References

This product has been referenced in:

  • Tremblay N  et al. Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response. PLoS Pathog 12:e1005772 (2016). WB . Read more (PubMed: 27454487) »
  • Sundaramoorthy S  et al. Functional genomics identifies a requirement of pre-mRNA splicing factors for sister chromatid cohesion. EMBO J N/A:N/A (2014). WB . Read more (PubMed: 25257310) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Cell (293T)
Specification
293T
Permeabilization
Yes - 0.5% Triton X100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 13 2015

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Nov 18 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 04 2013

Answer

Thank you for your response. Unfortunately, we do not have the pdf format of our on-line product datasheets yet but IT Department will consider implementing it. I have saved the on-line documents as word documents and attached the files to my response. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for your enquiry and your interest. The datasheets of these products can be found at our website: https://www.abcam.com/fibrillarin-antibody-nucleolar-marker-ab5821.html https://www.abcam.com/nop5p-nop56p-antibody-37c12-ab30310.html https://www.abcam.com/nop58-antibody-ab95315.html https://www.abcam.com/nhp2l1-antibody-ab95958.html If you need any further assistance in the future, please do not hesitate to contact me.

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