Key features and details
- Rabbit polyclonal to NHP2L1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NHP2L1 antibody
See all NHP2L1 primary antibodies
DescriptionRabbit polyclonal to NHP2L1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Zebrafish
Recombinant fragment corresponding to Human NHP2L1 aa 1-98.
Database link: P55769
- WB: HeLa and HepG2 whole cell lysates ICC/IF: Hela cell IHC-P: Cal27 xenograft
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab95958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 14 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/200.|
RelevanceOriginally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri snRNP. It binds to the 5' stem loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene.
Cellular localizationNucleus › nucleolus. Note: Concentrated in the dense fibrillar component of the nucleolus.
- [U4/U6.U5] tri snRNP antibody
- 15.5 kD RNA binding protein antibody
- High mobility group like nuclear protein 2 homolog 1 antibody
All lanes : Anti-NHP2L1 antibody (ab95958) at 1/1000 dilution
Lane 1 : Hela whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa
12% SDS PAGE
ab95958, at a 1/200 dilution, staining NHP2L1 in paraformaldehyde fixed HeLa cell by Immunofluorescence analysis.
ab95958, at a 1/100 dilution, staining NHP2L1 in paraffin embedded Cal27 xenograft by Immunohistochemistry.
ab95958 has been referenced in 2 publications.
- Tremblay N et al. Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response. PLoS Pathog 12:e1005772 (2016). WB . PubMed: 27454487
- Sundaramoorthy S et al. Functional genomics identifies a requirement of pre-mRNA splicing factors for sister chromatid cohesion. EMBO J N/A:N/A (2014). WB . PubMed: 25257310