The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri snRNP. It binds to the 5' stem loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene.
Nucleus › nucleolus. Note: Concentrated in the dense fibrillar component of the nucleolus.
Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labeling NHP2L1 with ab181982 at 1/1000 dilution followed by secondary Ready to use HRP Polymer for Rabbit IgG and hematoxylin counterstain.
Flow cytometry analysis of MCF-7 cells labeling NHP2L1 using ab181982 at 1/50 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.