Product nameNi-NTA Affinity Resin - Amintra
Ni- NTA Affinity Resin - Amintra (ab270549) is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Metal chelate affinity chromatography is a rapid one-step purification, which removes most contaminants and can achieve purities close to homogeneity. The rapid purification protocols provided in this handbook for affinity chromatography permit the recovery of high levels of pure recombinant protein in minutes. Large numbers of samples can be processed at the same time. Recombinant proteins purified using Ni- NTA Affinity Resin - Amintra may be used in a wide range of structure and activity-based laboratory procedures.
IMAC technology was introduced by Porath et al (1975). The matrix is attached to chelating groups that immobilize transition metal ions such as Ni2+, Co2+, Cu2+, Zn2+ (Porath and Olin, 1983; Porath, 1988; Sulkowski, 1989). Certain amino acids such as histidine, tryptophan, cysteine and tyrosine can act as electron donors on the surface of the protein and bind reversibly to the transition metal ion. In the vast majority of instances, 6x histidine tag is engineered at the N or C terminus of the protein (Kd-10-13 at pH 8.0).
Ni2+ is the most widely used metal ion as most IMAC tags seem to have very high affinity for immobilized Ni2+. The simplicity of IMAC technology is extremely attractive as it lends itself to the bind, wash and elute mode of operation if the appropriate buffer formulations are selected. IMAC can often be used with samples without any pre-treatment e.g. buffer exchange step. The use of metal chelate affinity is widespread for the selective adsorption of engineered recombinant proteins and has largely superseded non-affinity methods of chromatography for purifying
Characteristics of the resin:
Highle cross-lined 6% agarose
Charged metal ion
Bead size range
Recommended working pH
Static binding capacity
>40 mg 6x His-tagged
0.3 MPa (3 bar)
Solubility in water
This product is manufactured by Expedeon, an Abcam company and was previously called Amintra Ni-NTA Affinity Resin. Expedeon product codes ANN0010 (10 mL medium), ANN0025 (25 mL medium) and ANN0100 (100 mL) medium are the same as the current sizes: 10 mL, 25 mL and 100 mL respectively.
Storage instructionsShipped at Room Temperature. Store at +4°C. Please see notes section.
Storage bufferpH: 6
Constituents: Agarose, 20% Ethanol
Concentration information loading...
ab270549 has been referenced in 10 publications.
- Taylor RL et al. Loss-of-Function Mutations in the CFH Gene Affecting Alternatively Encoded Factor H-like 1 Protein Cause Dominant Early-Onset Macular Drusen. Ophthalmology 126:1410-1421 (2019). PubMed: 30905644
- Peters DT et al. Engineered mosaic protein polymers; a simple route to multifunctional biomaterials. J Biol Eng 13:54 (2019). PubMed: 31244892
- Rawlings AE et al. Artificial coiled coil biomineralisation protein for the synthesis of magnetic nanoparticles. Nat Commun 10:2873 (2019). PubMed: 31253765
- Sherlock D et al. Identification of the First Gene Transfer Agent (GTA) Small Terminase in Rhodobacter capsulatus and Its Role in GTA Production and Packaging of DNA. J Virol 93:N/A (2019). PubMed: 31534034
- Synakewicz M et al. Bioorthogonal protein-DNA conjugation methods for force spectroscopy. Sci Rep 9:13820 (2019). PubMed: 31554828
- Liao C et al. UCHL3 Regulates Topoisomerase-Induced Chromosomal Break Repair by Controlling TDP1 Proteostasis. Cell Rep 23:3352-3365 (2018). PubMed: 29898404
- De Cesare V et al. The MALDI-TOF E2/E3 Ligase Assay as Universal Tool for Drug Discovery in the Ubiquitin Pathway. Cell Chem Biol 25:1117-1127.e4 (2018). PubMed: 30017913
- Lommel M et al. Hydra Mesoglea Proteome Identifies Thrombospondin as a Conserved Component Active in Head Organizer Restriction. Sci Rep 8:11753 (2018). PubMed: 30082916
- Röttgerding F et al. Immune evasion of Borrelia miyamotoi: CbiA, a novel outer surface protein exhibiting complement binding and inactivating properties. Sci Rep 7:303 (2017). PubMed: 28331202
- Deshpande S et al. Thermostable exoshells fold and stabilize recombinant proteins. Nat Commun 8:1442 (2017). PubMed: 29129910