Product nameNi-NTA Affinity Resin - Amintra®
DescriptionNi-NTA Affinity Resin - Amintra®
Ni- NTA Affinity Resin - Amintra® (ab270549) is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Metal chelate affinity chromatography is a rapid one-step purification, which removes most contaminants and can achieve purities close to homogeneity. The rapid purification protocols provided in this handbook for affinity chromatography permit the recovery of high levels of pure recombinant protein in minutes. Large numbers of samples can be processed at the same time. Recombinant proteins purified using Ni- NTA Affinity Resin - Amintra® may be used in a wide range of structure and activity-based laboratory procedures.
This product is manufactured by Expedeon, an Abcam company. Expedeon product code ANN0010 was previously called Amintra® Ni-NTA Affinity Resin - 10 ml medium (20 ml in 50% slurry) and is the same as the 10 mL size of this product. Expedeon product code ANN0100 was previously called Amintra® Ni-NTA Affinity Resin - 100 ml medium (200 ml in 50% slurry) and is the same as the 100 mL size. Expedeon product code ANN0025 was previously called Amintra® Ni-NTA Affinity Resin - 25 ml medium (50 ml in 50% slurry) and is the same as the 25 mL size.
- Supporting matrix: Highly cross-linked 6% agarose
- Charged metal ion: Ni2+
- Bead size range: 45-165 µm
- Recommended working pH: pH 2.0-12.0
- Static binding capacity: >40mg 6x His-tagged
- Maximum pressure: 0.3MPa (3 bar)
- Chemical stability: High
- Solubility in water: Insoluble
Storage instructionsShipped at 4°C. Store at +4°C. Please see notes section.
Storage bufferConstituents: Agarose, 20% Ethanol
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ab270549 has been referenced in 10 publications.
- Taylor RL et al. Loss-of-Function Mutations in the CFH Gene Affecting Alternatively Encoded Factor H-like 1 Protein Cause Dominant Early-Onset Macular Drusen. Ophthalmology 126:1410-1421 (2019). PubMed: 30905644
- Peters DT et al. Engineered mosaic protein polymers; a simple route to multifunctional biomaterials. J Biol Eng 13:54 (2019). PubMed: 31244892
- Rawlings AE et al. Artificial coiled coil biomineralisation protein for the synthesis of magnetic nanoparticles. Nat Commun 10:2873 (2019). PubMed: 31253765
- Sherlock D et al. Identification of the First Gene Transfer Agent (GTA) Small Terminase in Rhodobacter capsulatus and Its Role in GTA Production and Packaging of DNA. J Virol 93:N/A (2019). PubMed: 31534034
- Synakewicz M et al. Bioorthogonal protein-DNA conjugation methods for force spectroscopy. Sci Rep 9:13820 (2019). PubMed: 31554828
- Liao C et al. UCHL3 Regulates Topoisomerase-Induced Chromosomal Break Repair by Controlling TDP1 Proteostasis. Cell Rep 23:3352-3365 (2018). PubMed: 29898404
- De Cesare V et al. The MALDI-TOF E2/E3 Ligase Assay as Universal Tool for Drug Discovery in the Ubiquitin Pathway. Cell Chem Biol 25:1117-1127.e4 (2018). PubMed: 30017913
- Lommel M et al. Hydra Mesoglea Proteome Identifies Thrombospondin as a Conserved Component Active in Head Organizer Restriction. Sci Rep 8:11753 (2018). PubMed: 30082916
- Röttgerding F et al. Immune evasion of Borrelia miyamotoi: CbiA, a novel outer surface protein exhibiting complement binding and inactivating properties. Sci Rep 7:303 (2017). PubMed: 28331202
- Deshpande S et al. Thermostable exoshells fold and stabilize recombinant proteins. Nat Commun 8:1442 (2017). PubMed: 29129910