Recombinant
RabMAb

Recombinant Anti-Nicalin antibody [EPR8528] (ab131159)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8528] to Nicalin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Nicalin antibody [EPR8528]
    See all Nicalin primary antibodies

  • Description

    Rabbit monoclonal [EPR8528] to Nicalin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human Nicalin aa 50-150.
    Database link: Q969V3

  • Positive control

    • A431, BxPC-3, 293T, C6, Raw 264.7 and NIH/3T3 cell lysates

  • General notes

     

     

    Previously labelled as NCLN.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab131159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-Nicalin antibody [EPR8528] (ab131159)
      Western blot - Anti-Nicalin antibody [EPR8528] (ab131159)
      All lanes : Anti-Nicalin antibody [EPR8528] (ab131159) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : BxPC-3 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : Raw 264.7 cell lysate
      Lane 6 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 63 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    • Other - Anti-Nicalin antibody [EPR8528] (ab131159)
      Other - Anti-Nicalin antibody [EPR8528] (ab131159)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References (0)

    Publishing research using ab131159? Please let us know so that we can cite the reference in this datasheet.

    ab131159 has not yet been referenced specifically in any publications.

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