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  1. Link

    nicalin-antibody-epr8528-ab131159.pdf

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Signal Transduction Growth Factors/Hormones TGF
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RecombinantRabMAb

Recombinant Anti-Nicalin antibody [EPR8528] (ab131159)

  • Datasheet
  • SDS
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Western blot - Anti-Nicalin antibody [EPR8528] (ab131159)
  • OI-RD Scanning - Anti-Nicalin antibody [EPR8528] (ab131159)
  • Anti-Nicalin antibody [EPR8528] (ab131159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8528] to Nicalin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Nicalin antibody [EPR8528]
    See all Nicalin primary antibodies
  • Description

    Rabbit monoclonal [EPR8528] to Nicalin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Nicalin aa 50-150.
    Database link: Q969V3

  • Positive control

    • A431, BxPC-3, 293T, C6, Raw 264.7 and NIH/3T3 cell lysates
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 8.10 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8528
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Developmental Biology
    • Embryogenesis
    • Axis formation and symmetry
    • Developmental Biology
    • Embryogenesis
    • Morphogens

Associated products

  • Alternative Versions

    • Anti-Nicalin antibody [EPR8528] - BSA and Azide free (ab248361)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.
  • Tissue specificity

    Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.
  • Sequence similarities

    Belongs to the nicastrin family.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession Q969V3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 56926 Human
    • Entrez Gene: 103425 Mouse
    • Entrez Gene: 314648 Rat
    • Omim: 609156 Human
    • SwissProt: Q969V3 Human
    • SwissProt: Q8VCM8 Mouse
    • SwissProt: Q5XIA1 Rat
    • Unigene: 657032 Human
    • Unigene: 302791 Mouse
    • Unigene: 4202 Rat
    see all
  • Alternative names

    • NCLN antibody
    • NCLN_HUMAN antibody
    • NET59 antibody
    • Nicalin antibody
    • Nicalin homolog (zebrafish) antibody
    • Nicastrin like protein antibody
    • Nicastrin-like protein antibody
    see all

Images

  • Western blot - Anti-Nicalin antibody [EPR8528] (ab131159)
    Western blot - Anti-Nicalin antibody [EPR8528] (ab131159)
    All lanes : Anti-Nicalin antibody [EPR8528] (ab131159) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : BxPC-3 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : Raw 264.7 cell lysate
    Lane 6 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 63 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-Nicalin antibody [EPR8528] (ab131159)
    OI-RD Scanning - Anti-Nicalin antibody [EPR8528] (ab131159)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Nicalin antibody [EPR8528] (ab131159)
    Anti-Nicalin antibody [EPR8528] (ab131159)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab131159? Please let us know so that we can cite the reference in this datasheet.

ab131159 has not yet been referenced specifically in any publications.

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