Recombinant Anti-Nicalin antibody [EPR8528] (ab131159)
![Western blot - Anti-Nicalin antibody [EPR8528] (ab131159)](https://www.abcam.com/ps/products/131/ab131159/Images/ab131159-145430-anti-nicalin-antibody-epr8528-western-blot.jpg "Western blot - Anti-Nicalin antibody [EPR8528] (ab131159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8528] to Nicalin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Nicalin antibody [EPR8528]
See all Nicalin primary antibodies -
Description
Rabbit monoclonal [EPR8528] to Nicalin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Nicalin aa 50-150.
Database link: Q969V3 -
Positive control
- A431, BxPC-3, 293T, C6, Raw 264.7 and NIH/3T3 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8528 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning. -
Tissue specificity
Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart. -
Sequence similarities
Belongs to the nicastrin family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 56926 Human
- Entrez Gene: 103425 Mouse
- Entrez Gene: 314648 Rat
- Omim: 609156 Human
- SwissProt: Q969V3 Human
- SwissProt: Q8VCM8 Mouse
- SwissProt: Q5XIA1 Rat
- Unigene: 657032 Human
see all -
Alternative names
- NCLN antibody
- NCLN_HUMAN antibody
- NET59 antibody
see all
Images
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Western blot - Anti-Nicalin antibody [EPR8528] (ab131159)All lanes : Anti-Nicalin antibody [EPR8528] (ab131159) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : C6 cell lysate
Lane 5 : Raw 264.7 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 63 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-Nicalin antibody [EPR8528] (ab131159)](https://www.abcam.com/ps/products/131/ab131159/Images/ab131159-145429-anti-nicalin-antibody-epr8528.jpg)
OI-RD Scanning - Anti-Nicalin antibody [EPR8528] (ab131159) -
![Anti-Nicalin antibody [EPR8528] (ab131159)](https://www.abcam.com/ps/products/131/ab131159/Images/ab131159-3-benefits-of-recombinant-antibodies.png)
Anti-Nicalin antibody [EPR8528] (ab131159)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131159 has not yet been referenced specifically in any publications.