Overview

  • Product name

    Anti-Nicalin antibody [EPR8528]
    See all Nicalin primary antibodies
  • Description

    Rabbit monoclonal [EPR8528] to Nicalin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Nicalin aa 50-150.
    Database link: Q969V3

  • Positive control

    • A431, BxPC-3, 293T, C6, Raw 264.7 and NIH/3T3 cell lysates
  • General notes

     

     

    Previously labelled as NCLN.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-Nicalin antibody [EPR8528] (ab131159) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : BxPC-3 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : Raw 264.7 cell lysate
      Lane 6 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 63 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131159 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131159.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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