Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8528] to Nicalin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Nicalin antibody [EPR8528]
See all Nicalin primary antibodies
DescriptionRabbit monoclonal [EPR8528] to Nicalin
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Nicalin aa 50-150.
Database link: Q969V3
- A431, BxPC-3, 293T, C6, Raw 264.7 and NIH/3T3 cell lysates
Previously labelled as NCLN.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 8.10 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).|
FunctionMay antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.
Tissue specificityHighly expressed in pancreas and skeletal muscle and, at lower levels, in heart.
Sequence similaritiesBelongs to the nicastrin family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- NCLN antibody
- NCLN_HUMAN antibody
- NET59 antibody
All lanes : Anti-Nicalin antibody [EPR8528] (ab131159) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : C6 cell lysate
Lane 5 : Raw 264.7 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 63 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131159 has not yet been referenced specifically in any publications.