Anti-Nicastrin antibody (ab45425)
Key features and details
- Rabbit polyclonal to Nicastrin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Nicastrin antibody
See all Nicastrin primary antibodies -
Description
Rabbit polyclonal to Nicastrin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Nicastrin.
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Positive control
- IHC-P: Human brain tissue. Human breast carcinoma tissue. ICC/IF: Human brain cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P | (1) |
Use a concentration of 2.5 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 2.5 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa. |
Target
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Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the nicastrin family. -
Cellular localization
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 23385 Human
- Omim: 605254 Human
- SwissProt: Q92542 Human
- Unigene: 517249 Human
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Alternative names
- Anterior pharynx defective 2 antibody
- APH 2 antibody
- APH2 antibody
see all
Images
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Lane 1 : Anti-Nicastrin antibody (ab45425) at 0.5 µg/ml
Lane 2 : Anti-Nicastrin antibody (ab45425) at 1 µg/ml
All lanes : Human brain tissue lysate
Predicted band size: 78 kDa -
ab45425 at 2.5 µg/ml staining Nicastrin in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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Immunofluorescence of Nicastrin in Human Brain cells using ab45425 at 20 ug/ml.
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ab45425 staining Nicastrin in Human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2500 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab45425 has been referenced in 4 publications.
- Takeichi T et al. A novel NCSTN missense mutation in the signal peptide domain causes hidradenitis suppurativa, which has features characteristic of an autoinflammatory keratinization disease. Br J Dermatol 182:491-493 (2020). PubMed: 31421058
- Bannai T et al. Chronic cerebral hypoperfusion shifts the equilibrium of amyloid ß oligomers to aggregation-prone species with higher molecular weight. Sci Rep 9:2827 (2019). PubMed: 30808940
- Lin Q et al. Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H. Int J Oncol 53:266-274 (2018). PubMed: 29749468
- D'Souza Y et al. Characterization of Aldh2 (-/-) mice as an age-related model of cognitive impairment and Alzheimer's disease. Mol Brain 8:27 (2015). WB, Dot blot ; Mouse . PubMed: 25910195