Product nameAnti-Nicastrin antibody [EPR2575Y]
See all Nicastrin primary antibodies
DescriptionRabbit monoclonal [EPR2575Y] to Nicastrin
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Nicastrin aa 650-750 (C terminal). The exact sequence is proprietary.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab68145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 100-120, 130-160 kDa (predicted molecular weight: 78 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionEssential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the nicastrin family.
Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Anterior pharynx defective 2 antibody
- APH 2 antibody
- APH2 antibody
All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HL-60 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 78 kDa
Additional bands at: 100-120 kDa (possible isoform), 130-160 kDa (possible isoform)
ab68145 has not yet been referenced specifically in any publications.