Recombinant
RabMAb

Recombinant Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free (ab247362)

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Overview

  • Product name

    Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free
    See all Nicastrin primary antibodies

  • Description

    Rabbit monoclonal [EPR2575Y] to Nicastrin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Nicastrin aa 650-750 (C terminal). The exact sequence is proprietary.

  • General notes

    Ab247362 is the carrier-free version of ab68145. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247362 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.

Actual band: 100-120, 130-160
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Belongs to the nicastrin family.

    • Cellular localization

      Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Anterior pharynx defective 2 antibody
      • APH 2 antibody
      • APH2 antibody
      • ATAG1874 antibody
      • KIAA0253 antibody
      • Ncstn antibody
      • NCT antibody
      • NICA_HUMAN antibody
      • Nicastrin antibody
      • RP11 517F10.1 antibody
      • RP11517F101 antibody
      see all

    References

    ab247362 has not yet been referenced specifically in any publications.

    Publishing research using ab247362? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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