Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
Key features and details
- Mouse monoclonal [6D3A10] to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10]
See all Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 primary antibodies -
Description
Mouse monoclonal [6D3A10] to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 aa 32-240. (Expressed in E.coli).
Database link: CHRNA3 -
Positive control
- WB: Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein; Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa and SMMC-7721 cells. Flow: SH-SY5Y and SK-N-SH cells.
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General notes
This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6D3A10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab233717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 57 kDa. | |
| Flow Cyt | 1/200 - 1/400. | |
| ICC/IF | 1/200 - 1/1000. |
Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1136 Human
- Omim: 118503 Human
- SwissProt: P32297 Human
- Unigene: 89605 Human
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Alternative names
- ACHA3_HUMAN antibody
- AChR antibody
- Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
see all
Images
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![Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)](https://www.abcam.com/ps/products/233/ab233717/Images/ab233717-307054-19.jpg)
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution + Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein
Predicted band size: 57 kDaExpected MW is 50.6 kDa.
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![Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)](https://www.abcam.com/ps/products/233/ab233717/Images/ab233717-307055-20.jpg)
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate.
Predicted band size: 57 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)](https://www.abcam.com/ps/products/233/ab233717/Images/ab233717-307056-21.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. -
![Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)](https://www.abcam.com/ps/products/233/ab233717/Images/ab233717-307057-22.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)SMMC-7721 (human hepatocarcinoma cell line) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. -
![Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)](https://www.abcam.com/ps/products/233/ab233717/Images/ab233717-307059-24.jpg)
Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cells labeling Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution (green) compared with a negative control (red).
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![Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)](https://www.abcam.com/ps/products/233/ab233717/Images/ab233717-307058-23.jpg)
Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Nicotinic Acetylcholine Receptor alpha using ab233717 at 1/200 dilution (green) compared to a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab233717 has not yet been referenced specifically in any publications.
