Overview

  • Product name

    Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10]
    See all Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 primary antibodies
  • Description

    Mouse monoclonal [6D3A10] to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 aa 32-240. (Expressed in E.coli).
    Database link: CHRNA3

  • Positive control

    • WB: Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein; Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa and SMMC-7721 cells. Flow: SH-SY5Y and SK-N-SH cells.
  • General notes

     This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 3

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    6D3A10
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 57 kDa.
Flow Cyt 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACHA3_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
    • Cholinergic receptor nicotinic alpha 3 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 3 antibody
    • CHRNA 3 antibody
    • CHRNA3 antibody
    • LNCR2 antibody
    • MGC104879 antibody
    • NACHRA 3 antibody
    • NACHRA3 antibody
    • Neuronal acetylcholine receptor protein alpha 3 chain precursor antibody
    • Neuronal acetylcholine receptor subunit alpha 3 antibody
    • Neuronal acetylcholine receptor subunit alpha-3 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 3 subunit antibody
    • PAOD2 antibody
    see all

Images

  • Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution + Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein

    Predicted band size: 57 kDa



    Expected MW is 50.6 kDa.

  • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
    Lane 2 : Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate.

    Predicted band size: 57 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
    Blue:  DRAQ5 fluorescent DNA dye.
    Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • SMMC-7721 (human hepatocarcinoma cell line) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
    Blue:  DRAQ5 fluorescent DNA dye.
    Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cells labeling Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution (green) compared with a negative control (red).

  • Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Nicotinic Acetylcholine Receptor alpha using ab233717 at 1/200 dilution (green) compared to a negative control (red).

References

ab233717 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233717.
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