Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
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Overview
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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10]
See all Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 primary antibodies -
Description
Mouse monoclonal [6D3A10] to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 aa 32-240. (Expressed in E.coli).
Database link: CHRNA3 -
Positive control
- WB: Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein; Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa and SMMC-7721 cells. Flow: SH-SY5Y and SK-N-SH cells.
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General notes
This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6D3A10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab233717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/2000. Predicted molecular weight: 57 kDa. | |
Flow Cyt | 1/200 - 1/400. | |
ICC/IF | 1/200 - 1/1000. |
Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1136 Human
- Omim: 118503 Human
- SwissProt: P32297 Human
- Unigene: 89605 Human
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Alternative names
- ACHA3_HUMAN antibody
- AChR antibody
- Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
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Images
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Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution + Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein
Predicted band size: 57 kDaExpected MW is 50.6 kDa.
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All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate.
Predicted band size: 57 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. -
Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
SMMC-7721 (human hepatocarcinoma cell line) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. -
Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cells labeling Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution (green) compared with a negative control (red).
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Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Nicotinic Acetylcholine Receptor alpha using ab233717 at 1/200 dilution (green) compared to a negative control (red).
Datasheets and documents
References
ab233717 has not yet been referenced specifically in any publications.