Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

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Overview

  • Product name

    Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10]
    See all Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 primary antibodies

  • Description

    Mouse monoclonal [6D3A10] to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 aa 32-240. (Expressed in E.coli).
    Database link: CHRNA3

  • Positive control

    • WB: Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein; Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa and SMMC-7721 cells. Flow: SH-SY5Y and SK-N-SH cells.

  • General notes

     This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 3

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    6D3A10

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 57 kDa.
Flow Cyt 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily.

  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ACHA3_HUMAN antibody
      • AChR antibody
      • Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
      • Cholinergic receptor nicotinic alpha 3 antibody
      • Cholinergic receptor nicotinic alpha polypeptide 3 antibody
      • CHRNA 3 antibody
      • CHRNA3 antibody
      • LNCR2 antibody
      • MGC104879 antibody
      • NACHRA 3 antibody
      • NACHRA3 antibody
      • Neuronal acetylcholine receptor protein alpha 3 chain precursor antibody
      • Neuronal acetylcholine receptor subunit alpha 3 antibody
      • Neuronal acetylcholine receptor subunit alpha-3 antibody
      • Neuronal nicotinic acetylcholine receptor alpha 3 subunit antibody
      • PAOD2 antibody
      see all

    Images

    • Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
      Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
      Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution + Human recombinant Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240) protein

      Predicted band size: 57 kDa



      Expected MW is 50.6 kDa.

    • Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
      Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
      All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717) at 1/500 dilution

      Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
      Lane 2 : Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 (aa32-240)-hIgGFc transfected HEK-293 cell lysate.

      Predicted band size: 57 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
      Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

      HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
      Blue:  DRAQ5 fluorescent DNA dye.
      Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

    • Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
      Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

      SMMC-7721 (human hepatocarcinoma cell line) cells stained for Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution in ICC/IF.
      Blue:  DRAQ5 fluorescent DNA dye.
      Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

    • Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
      Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

      Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cells labeling Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 using ab233717 at 1/200 dilution (green) compared with a negative control (red).

    • Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
      Flow Cytometry - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)

      Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Nicotinic Acetylcholine Receptor alpha using ab233717 at 1/200 dilution (green) compared to a negative control (red).

    References

    ab233717 has not yet been referenced specifically in any publications.

    Publishing research using ab233717? Please let us know so that we can cite the reference in this datasheet.

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