• Product name

    Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody
    See all Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 primary antibodies
  • Description

    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 aa 350-450 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab168251)

  • Positive control

    • This antibody gave a positive signal in Human Spinal Cord tissue lysate.
  • General notes

     This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 3



  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab110801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).


  • Function

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACHA3_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
    • Cholinergic receptor nicotinic alpha 3 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 3 antibody
    • CHRNA 3 antibody
    • CHRNA3 antibody
    • LNCR2 antibody
    • MGC104879 antibody
    • NACHRA 3 antibody
    • NACHRA3 antibody
    • Neuronal acetylcholine receptor protein alpha 3 chain precursor antibody
    • Neuronal acetylcholine receptor subunit alpha 3 antibody
    • Neuronal acetylcholine receptor subunit alpha-3 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 3 subunit antibody
    • PAOD2 antibody
    see all


  • Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab110801) at 1 µg/ml + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa
    Additional bands at: 51 kDa (possible cleavage fragment)

    Exposure time: 8 minutes

    The band observed at 51 kDa could potentially be a cleaved form of v due to the presence of a 31 amino acid signal peptide. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab110801 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.


This product has been referenced in:

  • Armstrong LC  et al. High-Throughput Patch Clamp Screening in Human a6-Containing Nicotinic Acetylcholine Receptors. SLAS Discov 22:686-695 (2017). Read more (PubMed: 28298165) »
See 1 Publication for this product

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