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Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 4
Predicted to work with:
Mouse, Rat, Chicken, Cow, Zebrafish, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 74-344 of Human Nicotinic Acetylcholine Receptor alpha 4 (Uniprot ID: P43681).
293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; Human DLD1 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Involvement in disease
Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Information by UniProt
A4 nicotinic receptor antibody
Acetylcholine receptor alpha 4 neural antibody
Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab151596)
All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab151596) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : H1299 whole cell lysate Lane 3 : HepG2 whole cell lysate Lane 4 : MOLT4 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 70 kDa 7.5% SDS-PAGE.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody (ab151596)
Immunohistochemical analysis of paraffin-embedded Human DLD1 xenograft tissue labeling Nicotinic Acetylcholine Receptor alpha 4 with ab151596 at 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"