Key features and details
- Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody
See all Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 primary antibodies
DescriptionRabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish, Xenopus tropicalis
Recombinant fragment corresponding to Human Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 aa 74-344.
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; Human DLD1 xenograft tissue.
This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 4
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 70 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Involvement in diseaseDefects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- A4 nicotinic receptor antibody
- Acetylcholine receptor alpha 4 neural antibody
- Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab151596) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 70 kDa
Immunohistochemical analysis of paraffin-embedded Human DLD1 xenograft tissue labeling Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 with ab151596 at 1/100 dilution.
ab151596 has not yet been referenced specifically in any publications.