Overview

  • Product name

    Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody
    See all Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 primary antibodies
  • Description

    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4
  • Host species

    Rabbit
  • Specificity

    Antiserum Specificity Polypeptide % Cross Reactivity Nicotinic a4 receptor (620-627) 100 Nicotinic a4 receptor ~80 Nicotinic a3 receptor 0 Nicotinic a5 receptor 0 Nicotinic a7 receptor 0 Nicotinic ß2 receptor 0 Nicotinic ß3 receptor 0 Nicotinic ß4 receptor 0
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human, Chimpanzee, Pufferfish, Zebrafish, Snail
  • Immunogen

    Synthetic peptide corresponding to Human Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 aa 620-627 (C terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Sequence:

    PPWLAGMI


    (Peptide available as ab49523, ab99319)

  • General notes

     This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab41172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250.
WB 1/800. Predicted molecular weight: 70 kDa. With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC/IF 1/200.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/1000.

Target

  • Function

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in disease

    Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A4 nicotinic receptor antibody
    • Acetylcholine receptor alpha 4 neural antibody
    • Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
    • ACH 4 antibody
    • ACH4 antibody
    • ACHA4_HUMAN antibody
    • AChR antibody
    • Acra 4 antibody
    • Acra4 antibody
    • Alpha4 nAChR antibody
    • BFNC antibody
    • Cholinergic receptor nicotinic alpha 4 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 4 antibody
    • CHRNA 4 antibody
    • CHRNA4 antibody
    • EBN 1 antibody
    • EBN antibody
    • EBN1 antibody
    • NACHR antibody
    • NACRA 4 antibody
    • NACRA4 antibody
    • Neuronal acetylcholine receptor subunit alpha-4 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 4 subunit antibody
    see all

Images

  • All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172) at 1/800 dilution

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 70 kDa

  • ab41172 (1/250 dilution) staining Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and membrane compartment within the glia region of cells
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:

  • Chen N  et al. An acetylcholine-activated microcircuit drives temporal dynamics of cortical activity. Nat Neurosci 18:892-902 (2015). IHC . Read more (PubMed: 25915477) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 24°C
Sample
Snail (Helix pomatia) Tissue sections (brain)
Specification
brain
Permeabilization
Yes - TX
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 27 2013

Answer

Thank you for your reply.

As we have no sequence for the proteins from Helix pomatia I cannot predict how likely the antibodies you would like to try will cross react. We would therefore not usually offer the testing discount scheme for this. However I am willing to make an exception in this case if you would still like to try it. The testing discount codes for the two antibodies as follows:

ab10096, discount code: xxxxxx, expiration date: 13th March 2013, value: €380.00

ab41172, discount code: xxxxxx, expiration date: 13th March 2013, value: €370.00

The codes above will give you €380.00 and €370.00 respectively off your next order before the expiration date. To redeem this offer, please submit an Abreview for Helix pomatia in Western blotting and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site:

www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

www.abcam.com/abtrial

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Answer

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these additional two antibodies:
ab13898
ab28669
I hope this will be helpful. I look forward to receiving your confirmation that you would like to go ahead, and I will be pleased to arrange and send the discount codes to you.
For the remaining antibodies, I can suggest you may like to consider submitting an Abreview anyway, for which you can obtain Abpoints:
ab85612
ab75874
ab61113
ab47504
ab16476
ab85608
ab31232
ab41172
ab23832
Thank you for your time and continued interest in our products. I look forward to hearing from you.

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Answer

Thank you very much for your interest in our antibodies.

We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

If a customer would like to test an antibody in an application or species that it has not been previously tested in, we are able to provide our testing discount program.

In this case, the antibodiess you have listed have all been tested in western blot already, but we would be able to provide this offer to you to test the following antibodies in mouse and rat:

Discount to detect in mouse and rat (the following antibodies have not yet been tested in mouse or rat):
CDC42 Binding Protein Kinase Beta antibody ab61328
CNKR2 antibody ab64844
NEK7 antibody ab80948

Discount to test in mouse (the following antibodies have not yet been tested in mouse):
MEK3 antibody ab5428
BMX antibody ab73887
Muscarinic Acetylcholine Receptor 1 antibody ab75178

Discount to test in rat (the following antibodies have not been tested in rat):
PPP4C antibody sb70888

The following are already tested in western blot, and in mouse and/or rat as per your list. I am sorry we are therefore not able to provide a testing discount for these.
ROCK2 antibody ab85612 Already tested WB, ms and rat
CAMKIV antibody [EP2564Y] ab75874 Already tested WB, ms and rat
FAK antibody ab61113 Already tested WB, ms and rat
S6K antibody ab47504 Previously tested WB, ms and rat
PPP1A antibody ab 16476 Previously tested WB, ms and rat
5HT7 Receptor antibody ab13898 WB and mouse already tested.
NMDAR1 antibody ab28669 Previously tested WB, ms and rat
Dopamine Receptor D1 antibody ab85608 Previously tested WB, ms and rat
Glutamate Receptor 1 (AMPA subtype) antibody ab31232 Previously tested WB and rat
Nicotinic Acetylcholine Receptor alpha 4 antibody ab41172 Previously tested WB and mouse
Nicotinic Acetylcholine Receptor alpha 7 antibody ab23832 Previously tested WB and rat

For the antibodies we can provide a discount for, I can offer a discount off a future purchase if you buy the antibody(ies) now, test them in mouse, rat or both as indicated above, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount provided for each individual antibody would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test these antibodies in mouse, rat or both. I will then send a discount code. This code must be issued before purchasing the antibodies so please wait for my reply before ordering.

2. Purchase the antibodies either by phone, fax, or online (www.abcam.com).

3. Test it in mouse, rat or both.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN] ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for the species, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Also, we are able to provide a discount for any order of 5 items or more. Therefore, once you have decided which antibodies you are going to purchase, please contact our customer service department before you place the order with a full list of the antibodies you wish to buy (orders@abcam.com). They will then be able to provide you with a quote.

I hope this will be helpful to you. I look forward to receiving confirmation that you would like to receive the discount codes. If you have any further questions, please do not hesitate to contact me.

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