Recombinant
Recombinant Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (ab124832)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)]
See all Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 primary antibodies -
Description
Rabbit monoclonal [EPR4563(2)] to Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 aa 500-600. The exact sequence is proprietary.
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Positive control
- SH-SY5Y, A673, C6 and Neuro-2a cell lysates.
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General notes
This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 4
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4563(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt,ICC or IHC-P.
Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
Involvement in disease
Defects in CHRNA4 are the cause of nocturnal frontal lobe epilepsy type 1 (ENFL1) [MIM:600513]; also symbolized ADNFLE. ENFL1 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1137 Human
- Entrez Gene: 11438 Mouse
- Entrez Gene: 25590 Rat
- Omim: 118504 Human
- SwissProt: P43681 Human
- SwissProt: O70174 Mouse
- SwissProt: P09483 Rat
- Unigene: 10734 Human
see all -
Alternative names
- A4 nicotinic receptor antibody
- Acetylcholine receptor alpha 4 neural antibody
- Acetylcholine receptor neuronal nicotinic alpha 4 subunit antibody
see all
Images
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![Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (ab124832)](https://www.abcam.com/ps/products/124/ab124832/Images/ab124832-138781-anti-nicotinic-acetylcholine-receptor-alpha-4-antibody-epr45632-western-blot.jpg)
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (ab124832)All lanes : Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (ab124832) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : A673 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Liu Y et al. Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets. Mar Drugs 17:N/A (2019). Read more (PubMed: 31035425) »
- Chen SP et al. Involvement of hippocampal acetylcholinergic receptors in electroacupuncture analgesia in neuropathic pain rats. Behav Brain Funct 12:13 (2016). Read more (PubMed: 27068709) »
