Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody (ab166718)
Key features and details
- Goat polyclonal to Nicotinic Acetylcholine Receptor alpha 5/CHRNA5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody
See all Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 primary antibodies -
Description
Goat polyclonal to Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chicken, Hamster, Cow, Pig, Chimpanzee, Monkey, Gorilla, Elephant -
Immunogen
Synthetic peptide corresponding to Human Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 aa 369-380 (internal sequence) (Cysteine residue).
Sequence:HVDRYFTQKEET
Database link: NP_000736.2 -
Positive control
- KELLY lysate; Human Colon, Submucosal Plexus tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 53 kDa.
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IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 53 kDa. |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-5/CHRNA5 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 386577 Chicken
- Entrez Gene: 619369 Chimpanzee
- Entrez Gene: 282177 Cow
- Entrez Gene: 1138 Human
- Omim: 118505 Human
- SwissProt: P26152 Chicken
- SwissProt: Q5IS51 Chimpanzee
- SwissProt: Q8SPU7 Cow
see all -
Alternative names
- Acetylcholine receptor nicotinic alpha 5 (neuronal) antibody
- ACHA5_HUMAN antibody
- AChR antibody
see all
Images
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Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody (ab166718) at 1 µg/ml + Kelly cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 53 kDa -
Immunohistochemical analysis of Formalin-Fixed, Paraffin-Embedded Human Colon, Submucosal Plexus tissue labeling Nicotinic Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 with ab166718 at 3.75 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab166718 has been referenced in 3 publications.
- Qi JC et al. Cholinergic a5 nicotinic receptor is involved in the proliferation and invasion of human prostate cancer cells. Oncol Rep 43:159-168 (2020). PubMed: 31789411
- Dang N et al. α5-nAChR modulates melanoma growth through the Notch1 signaling pathway. J Cell Physiol 235:7816-7826 (2020). PubMed: 31907929
- Jiang Y et al. TRPC channels mediated calcium entry is required for proliferation of human airway smooth muscle cells induced by nicotine-nAChR. Biochimie 158:139-148 (2018). PubMed: 30550855