Overview

  • Product name
    Anti-Nicotinic Acetylcholine Receptor alpha 5 antibody
    See all Nicotinic Acetylcholine Receptor alpha 5 primary antibodies
  • Description
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 5
  • Host species
    Rabbit
  • Specificity
    Antiserum Specificity Polypeptide % Cross Reactivity Nicotinic a5 receptor (460-468) 100%, Nicotinic a5 receptor ~95%, Nicotinic a3 receptor 0%, Nicotinic a4 receptor 0%, Nicotinic a7 receptor 0%, Nicotinic ß2 receptor 0%, Nicotinic ß3 receptor 0%, Nicotinic ß4 receptor 0%.
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human, Chimpanzee, Monkey
  • Immunogen

    Synthetic peptide:

    PVHIGNANK

    , corresponding to C terminal amino acids 460-468 of Nicotinic Acetylcholine Receptor alpha 5

Properties

Applications

Our Abpromise guarantee covers the use of ab41173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC/IF: 1/400.
    WB: 1/1600. Predicted molecular weight: 53 kDa.

    With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
    • Sequence similarities
      Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-5/CHRNA5 sub-subfamily.
    • Cellular localization
      Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acetylcholine receptor nicotinic alpha 5 (neuronal) antibody
      • ACHA5_HUMAN antibody
      • AChR antibody
      • Cholinergic receptor neuronal nicotinic alpha polypeptide 5 antibody
      • Cholinergic receptor nicotinic alpha 5 antibody
      • Cholinergic receptor nicotinic alpha polypeptide 5 antibody
      • CHRNA 5 antibody
      • CHRNA5 antibody
      • LNCR2 antibody
      • NACHRA 5 antibody
      • NACHRA5 antibody
      • Neuronal acetylcholine receptor subunit alpha 5 antibody
      • Neuronal acetylcholine receptor subunit alpha-5 antibody
      • Neuronal nicotinic acetylcholine receptor alpha 5 subunit antibody
      • Neuronal nicotinic acetylcholine receptor, alpha5 subunit antibody
      • OTTHUMP00000185019 antibody
      see all

    References

    This product has been referenced in:
    • Calleja-Macias IE  et al. Cholinergic signaling through nicotinic acetylcholine receptors stimulates the proliferation of cervical cancer cells: an explanation for the molecular role of tobacco smoking in cervical carcinogenesis? Int J Cancer 124:1090-6 (2009). WB ; Human . Read more (PubMed: 19048619) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for your message and for providing this further information. I appreciate the time your customer has spent on these experiments and would be pleased to arrange a replacement or a credit note in compensation. I look forward to hearing from you with details of how your customer would like to proceed.  

    Read More

    Answer

    Thank you for your cooperation. As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order. As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. The reference number, or credit ID, is *******. Please refer to this number in any correspondence with the accounting department. I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

    Read More

    Question

    LOT NUMBER gr7502-1 ORDER NUMBER *********** DESCRIPTION OF THE PROBLEM Multiple bands,high background, and didn't have the correct band. SAMPLE * Species:Human * What’s cell line or tissue:Human Lung cancer cell lines, including HCC827 A549 H1975 H2170 etc * Cell extract or Nuclear extract:Total cell lysis * Purified protein or Recombinant protein: Endogenouspurified protein PRIMARY ANTIBODY Species:Rabbit Reacts against:Human * At what dilution(s) have you tested this antibody:1:1600 * What dilution buffer was used: As well as blocking buffer * Incubation time:RT, 1 hour * Incubation temperature:RT * What washing steps were done:3 times in TBST, each time 10 min DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED A549 and HCC827 cell lines were our positive control. HCC827 knockdown nAChR-α5 (si-α5) and H520 cell lines were our negative control. ANTIBODY STORAGE CONDITIONS -20oC SAMPLE PREPARATION What lysis buffer was used: RIPA What protease inhibitors were used:•Roche complete, EDTA-Free What loading buffer was used:50 mMTris-HCl pH 6.8 2% SDS 10% glycerol 1% β-mercaptoethanol 12.5 mM EDTA 0.02 % bromophenol blue Phosphatase inhibitors :Halt Phosphatase Inhibitor Cocktailpurchase from Thermo Did you heat the samples: temperature and time:Yes, 99oC, 5 min AMOUNT OF PROTEIN LOADED 100 ug ELECTROPHORESIS/GEL CONDITIONS Reducing or non reducing gel: SDS-PAGE gell Reducing agent:SDS Gel percentage :8% TRANSFER AND BLOCKING CONDITIONS Transfer conditions: (Type of membrane, Protein transfer verified): Amersham ECL gel membrane, 100% transfer to membrane. Buffer:TBST Blocking agent: milk, BSA, serum, what percentage:2% silk milk Incubation time:RT 1 hour or 4oC overnight Incubation temperature:RT SECONDARY ANTIBODY Species:Goat Reacts against:Rabbit At what dilution(s) have you tested this antibody: •1:10000 Incubation time:RT, 1 hour Wash steps:3 times in TBST, each time 10 min Fluorochrome or enzyme conjugate:HRP (enzyme conjugate) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? concentration of primary antibody

    Read More
    Answer

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab4117 : - I would suggest to load not more than 40 µg on the gel. An excess of sample often brings high background and a lack of specificity for both primary and secondary antibodies. - I would also recommend to use 5% BSA as blocking agent (or 5% milk) and to block the membrane for 1 or 2 hours at room temperature. Use this blocking solution for the incubation of the primary. - An incubation of the primary can be done either at room temperature for 2 hours or at 4°C ovenight. The best conditions may vary from one antibody to another. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up