Key features and details
- Goat polyclonal to Nicotinic Acetylcholine Receptor alpha 5/CHRNA5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody
DescriptionGoat polyclonal to Nicotinic Acetylcholine Receptor alpha 5/CHRNA5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Chicken, Hamster, Cow, Pig, Chimpanzee, Monkey, Gorilla, Elephant
- KELLY lysate; Human Colon, Submucosal Plexus tissue.
This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 5
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab166718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 53 kDa.|
|IHC-P||Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-5/CHRNA5 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- Acetylcholine receptor nicotinic alpha 5 (neuronal) antibody
- ACHA5_HUMAN antibody
- AChR antibody
Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody (ab166718) at 1 µg/ml + Kelly cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Immunohistochemical analysis of Formalin-Fixed, Paraffin-Embedded Human Colon, Submucosal Plexus tissue labeling Nicotinic Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 with ab166718 at 3.75 µg/ml.
ab166718 has been referenced in 1 publication.
- Jiang Y et al. TRPC channels mediated calcium entry is required for proliferation of human airway smooth muscle cells induced by nicotine-nAChR. Biochimie 158:139-148 (2018). PubMed: 30550855