Overview

  • Product name

    Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody
    See all Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 primary antibodies
  • Description

    Goat polyclonal to Nicotinic Acetylcholine Receptor alpha 5/CHRNA5
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Chicken, Hamster, Cow, Pig, Chimpanzee, Monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 aa 369-380 (internal sequence) (Cysteine residue).
    Sequence:

    HVDRYFTQKEET


    Database link: NP_000736.2

  • Positive control

    • KELLY lysate; Human Colon, Submucosal Plexus tissue.
  • General notes

     This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab166718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 53 kDa.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-5/CHRNA5 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acetylcholine receptor nicotinic alpha 5 (neuronal) antibody
    • ACHA5_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor neuronal nicotinic alpha polypeptide 5 antibody
    • Cholinergic receptor nicotinic alpha 5 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 5 antibody
    • CHRNA 5 antibody
    • CHRNA5 antibody
    • LNCR2 antibody
    • NACHRA 5 antibody
    • NACHRA5 antibody
    • Neuronal acetylcholine receptor subunit alpha 5 antibody
    • Neuronal acetylcholine receptor subunit alpha-5 antibody
    • Neuronal nicotinic acetylcholine receptor alpha 5 subunit antibody
    • Neuronal nicotinic acetylcholine receptor, alpha5 subunit antibody
    • OTTHUMP00000185019 antibody
    see all

Images

  • Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 antibody (ab166718) at 1 µg/ml + Kelly cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 53 kDa

  • Immunohistochemical analysis of Formalin-Fixed, Paraffin-Embedded Human Colon, Submucosal Plexus tissue labeling Nicotinic Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 with ab166718 at 3.75 µg/ml.

References

This product has been referenced in:

  • Jiang Y  et al. TRPC channels mediated calcium entry is required for proliferation of human airway smooth muscle cells induced by nicotine-nAChR. Biochimie 158:139-148 (2018). Read more (PubMed: 30550855) »
See 1 Publication for this product

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There are currently no Customer reviews or Questions for ab166718.
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