Overview

  • Product name
    Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody
    See all Nicotinic Acetylcholine Receptor alpha 7 primary antibodies
  • Description
    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7
  • Host species
    Rabbit
  • Specificity
    Several significant splice variants of the protein have been reported, but currently, we do not know, which variants are detected by this antibody.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH, selected from within the amino acid range 22-71 of Human Nicotinic Acetylcholine Receptor alpha 7. (Peptide available as ab101467.)

  • Positive control
    • HepG2 lysate and Mouse muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab10096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Can be blocked with Human Nicotinic Acetylcholine Receptor alpha 7 peptide (ab101467). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 7nAChR antibody
    • a7 nicotinic acetylcholine receptor antibody
    • acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
    • ACHA7_HUMAN antibody
    • AChR antibody
    • Acra7 antibody
    • alpha 7 neuronal nicotinic acetylcholine receptor antibody
    • alpha-7 nicotinic cholinergic receptor subunit antibody
    • alpha7 antibody
    • cholinergic receptor, neuronal nicotinic, alpha polypeptide 7 antibody
    • cholinergic receptor, nicotinic, alpha 7 (neuronal) antibody
    • cholinergic receptor, nicotinic, alpha 7 antibody
    • cholinergic receptor, nicotinic, alpha polypeptide 7 antibody
    • CHRNA7 antibody
    • CHRNA7-2 antibody
    • NACHRA7 antibody
    • neuronal acetylcholine receptor protein, alpha-7 chain antibody
    • Neuronal acetylcholine receptor subunit alpha-7 antibody
    see all

Images

  • Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab10096) at 2.5 µg/ml + HepG2 cell lysate

    Predicted band size: 56 kDa

  • NNK (NCI) (100 mg/kg) was administered to BALB/c mice. The mice were randomized into two groups; group one received the vehicle (PBS) (n = 8) and group two received nicotine (n = 8) by i.p. injection at a dose of 1 mg/kg three times a week for 28 weeks. Lungs from the mice were fixed in 10% neutral-buffered formalin before processing into paraffin blocks. Sections were subjected to microwave ‘antigen retrieval’ for 20 minutes on 70% power, with a 1 minute cooling period after every 5 minutes, in 0.01 M sodium citrate, pH 6.0. Slides were stained either with H&E alone or with ab10096 against α7 nAChR (1∶50 dilution). For color development the slides were treated with peroxidase substrate kit from Vector labs and developed using DAB as chromogen. After a final rinse in dH2O, sections were lightly counterstained in hematoxylin, dehydrated and mounted.

  • WB using ab10096. Blots were incubated with ab10096 for at least 1 hour and for 30-90 minutes for the secondary antibody. 3% BSA and 10% FBS was used as blocking solution. anti- bungarotoxin was added in excess before precipitation in lanes 2 and 4. Nicotinic acetylcholine receptor alpha 7 precipitates as a double band (as shown by Drisdel and Green, 2000). The higher bands (79-122 kD) are probably dimeric and trimeric forms of the protein.  Review by Andreas Wiesner submitted 7 May 2004.
      WB using ab10096. Blots were incubated with ab10096 for at least 1 hour and for 30-90 minutes for the secondary antibody. 3% BSA and 10% FBS was used as blocking solution. anti- bungarotoxin was added in excess before precipitation in lanes 2 and 4. Nicotinic acetylcholine receptor alpha 7 precipitates as a double band (as shown by Drisdel and Green, 2000). The higher bands (79-122 kD) are probably dimeric and trimeric forms of the protein. Review by Andreas Wiesner submitted 7 May 200

References

This product has been referenced in:
  • Jiang P  et al. Astragaloside IV Prevents Obesity-Associated Hypertension by Improving Pro-Inflammatory Reaction and Leptin Resistance. Mol Cells 41:244-255 (2018). Read more (PubMed: 29562733) »
  • Foucault-Fruchard L  et al. Alpha-7 nicotinic acetylcholine receptor agonist treatment in a rat model of Huntington's disease and involvement of heme oxygenase-1. Neural Regen Res 13:737-741 (2018). Read more (PubMed: 29722329) »
See all 31 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
ELISA
Sample
Human Recombinant protein (alpha7 extracellular domain)
Specification
alpha7 extracellular domain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Type
Sandwich (Detection)

Abcam user community

Verified customer

Submitted Jun 01 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4µg/mL · Temperature: 27°C

Rita Machaalani

Verified customer

Submitted May 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (muscle)
Loading amount
10 µg
Specification
muscle
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 18 2013

Question
Answer

That is no problem. I hope they work well for you.

If I can be of any further assistance, please do not hesitate to let me know.

Until then, I wish you all the best with your research.

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Answer

Thank you for your reply.

As we have no sequence for the proteins from Helix pomatia I cannot predict how likely the antibodies you would like to try will cross react. We would therefore not usually offer the testing discount scheme for this. However I am willing to make an exception in this case if you would still like to try it. The testing discount codes for the two antibodies as follows:

ab10096, discount code: xxxxxx, expiration date: 13th March 2013, value: €380.00

ab41172, discount code: xxxxxx, expiration date: 13th March 2013, value: €370.00

The codes above will give you €380.00 and €370.00 respectively off your next order before the expiration date. To redeem this offer, please submit an Abreview for Helix pomatia in Western blotting and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site:

www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

www.abcam.com/abtrial

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Answer

Vielen Dank für ihren Anruf. Bitte entschuldigen Sie die Verzögerung. ich habe noch auf Immunogen Informationen aus dem herstellenden Labor gewartet.

Ich freue mich Ihnen bestätigen zu können, dass vier der fünf Abcam Antikörper sehr wahrscheinlich mit den angegeben Spezies und in nativem WB funktionieren.

Bitte beachten Sie, dass die anderen Antikörper von einem anderen Unternehmen stammen. Bitte lassen Sie mich wissen, ob Sie an alternativen Antikörpern von Abcam interessiert sind und ich werde Ihnen gerne helfen, die passenden Antikörper herauszusuchen.

ab10096 : Homologie des Immunogens mit Kaninchen nur 46%. Ich kann deshalb diesen Antikörper nicht empfehlen für Ihre Versuche!
ab31232 : Homologie des Immunogens mit Kaninchen 100%
ab85608 : Homologie des Immunogens mit Kaninchen 100%
ab106515 : Maus ist getestet und garantiert
ab134308 : Maus ist getestet und garantiert


Keiner dieser Antikörper hat einen Abbildung von nativen WB.

Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat und Abbildung zusenden. Der Rabatt würde den ganzen Wert vom getesteten Antikörper abdecken, und gegen eine erneute Bestellung weiterer Produkte von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie welche/n Antikörper Sie testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in der entsprechenden Spezies und in nativem WB.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können weitere Produkte bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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Answer

Thank you for forwarding the complaint and for providing more information about your customer's protocol. Is this the same customer who is having problem with three of our antibodies at the same time? One of our collaborators has tested this antibody for us and he has also submitted a positive review. He has used this antibody on formalin-fixed samples but not on acetone-fixed cells so we do not know if it works on frozen sections at all. Please refer your customer to this scientist’s review which can be found on-line, his name is Andreas Wiesner. He obtained comparable staining using primary neuronal hippocampus cell cultures and with transfixed and permeabilised COS-cells. For staining experiments SHSY5Y-cells were costained with rhodamine coupled á-btx and ab10096 for 60 minutes. Fixation was done in 4% PFA for 15 minutes. Permeabilisation if necessary was performed with 0.002% Triton-X for 4 minutes. Ab 10096 was used at a dilution of 1:1000 and secondary antibodies at 1:200. A FITC coupled secondary antibody against rabbit was used. We would like to emphasize the fact that the target is an integral membrane protein, therefore we would suggest solubilizing the membrane with Triton before the staining procedure. We hope this will be of some help.

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