Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab110851)
Key features and details
- Goat polyclonal to Nicotinic Acetylcholine Receptor alpha 7
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody
See all Nicotinic Acetylcholine Receptor alpha 7 primary antibodies -
Description
Goat polyclonal to Nicotinic Acetylcholine Receptor alpha 7 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to Human Nicotinic Acetylcholine Receptor alpha 7 aa 300-400.
Database link: P36544 -
Positive control
- Human Brain cortex tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99.48% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| All applications |
Mouse
Rat
Chicken
Cow
Dog
Pig
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-P
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 374001 Chicken
- Entrez Gene: 282178 Cow
- Entrez Gene: 488696 Dog
- Entrez Gene: 1139 Human
- Entrez Gene: 11441 Mouse
- Entrez Gene: 100415931 Pig
- Entrez Gene: 25302 Rat
- Omim: 118511 Human
see all -
Alternative names
- 7nAChR antibody
- a7 nicotinic acetylcholine receptor antibody
- acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab110851)ab110851 at 5µg/ml staining Nicotinic Acetylcholine Receptor alpha 7 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
References (1)
ab110851 has been referenced in 1 publication.
- Nunes NS et al. Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathway. EBioMedicine 45:495-510 (2019). PubMed: 31253515
