Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody
See all Nicotinic Acetylcholine Receptor alpha 7 primary antibodies -
Description
Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Nicotinic Acetylcholine Receptor alpha 7 aa 491-502 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:PNFVEAVSKDFA
Database link: P36544 -
Positive control
- ICC/IF: HEK 293T cells. IHC-P: Mouse brain tissues and human kidney tissue. WB: mouse brain lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab216485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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| WB | 1/100 - 1/1000. Predicted molecular weight: 56 kDa. | |
| ICC/IF | 1/500. |
Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1139 Human
- Entrez Gene: 11441 Mouse
- Omim: 118511 Human
- SwissProt: P36544 Human
- SwissProt: P49582 Mouse
- Unigene: 511772 Human
- Unigene: 113464 Mouse
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Alternative names
- 7nAChR antibody
- a7 nicotinic acetylcholine receptor antibody
- acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
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Images
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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485) at 1/300 dilution + mouse brain lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/500 dilution
Predicted band size: 56 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by Cy3 conjugated secondary Goat Anti-Rabbit IgG. DAPI was used to stain the cell nuclei.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse brain tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by secondary detection and DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)Immunofluorescent analysis of HEK293 cells labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/500 dilution, followed by secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated. DAPI staining of nuclei is shown too.
Protocols
Datasheets and documents
References
ab216485 has not yet been referenced specifically in any publications.
