Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
Key features and details
- Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody
See all Nicotinic Acetylcholine Receptor alpha 7 primary antibodies -
Description
Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Nicotinic Acetylcholine Receptor alpha 7 aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P36544 -
Positive control
- ICC/IF: HEK 293T cells. IHC-P: Mouse brain tissues and human kidney tissue. WB: mouse brain lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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| WB | (2) |
1/100 - 1/1000. Predicted molecular weight: 56 kDa.
|
| ICC/IF | (1) |
1/500.
|
| Notes |
|---|
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
|
WB
1/100 - 1/1000. Predicted molecular weight: 56 kDa. |
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ICC/IF
1/500. |
Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1139 Human
- Entrez Gene: 11441 Mouse
- Omim: 118511 Human
- SwissProt: P36544 Human
- SwissProt: P49582 Mouse
- Unigene: 511772 Human
- Unigene: 113464 Mouse
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Alternative names
- 7nAChR antibody
- a7 nicotinic acetylcholine receptor antibody
- acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
see all
Images
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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485) at 1/300 dilution + mouse brain lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/500 dilution
Predicted band size: 56 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by Cy3 conjugated secondary Goat Anti-Rabbit IgG. DAPI was used to stain the cell nuclei.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse brain tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by secondary detection and DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)Immunofluorescent analysis of HEK293 cells labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/500 dilution, followed by secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated. DAPI staining of nuclei is shown too.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab216485 has been referenced in 7 publications.
- Wang D et al. The Availability of the a7-Nicotinic Acetylcholine Receptor in Early Identification of Vulnerable Atherosclerotic Plaques: A Study Using a Novel 18F-Label Radioligand PET. Front Bioeng Biotechnol 9:640037 (2021). PubMed: 33777911
- Ni H et al. Nicotine Modulates CTSS (Cathepsin S) Synthesis and Secretion Through Regulating the Autophagy-Lysosomal Machinery in Atherosclerosis. Arterioscler Thromb Vasc Biol 40:2054-2069 (2020). PubMed: 32640907
- Dong Y et al. Nicotine Prevents Oxidative Stress-Induced Hippocampal Neuronal Injury Through a7-nAChR/Erk1/2 Signaling Pathway. Front Mol Neurosci 13:557647 (2020). PubMed: 33328880
- Xu H et al. Downregulation of a7 nicotinic acetylcholine receptors in peripheral blood monocytes is associated with enhanced inflammation in preeclampsia. BMC Pregnancy Childbirth 19:188 (2019). PubMed: 31138166
- Seoane-Collazo P et al. Central nicotine induces browning through hypothalamic ? opioid receptor. Nat Commun 10:4037 (2019). PubMed: 31492869
- Velazquez R et al. Lifelong choline supplementation ameliorates Alzheimer's disease pathology and associated cognitive deficits by attenuating microglia activation. Aging Cell 18:e13037 (2019). PubMed: 31560162
- Wang Q et al. Dysregulated repair and inflammatory responses by e-cigarette-derived inhaled nicotine and humectant propylene glycol in a sex-dependent manner in mouse lung. FASEB Bioadv 1:609-623 (2019). PubMed: 31825014