Key features and details
- Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Nicotinic Acetylcholine Receptor alpha 7 antibody
See all Nicotinic Acetylcholine Receptor alpha 7 primary antibodies
DescriptionRabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- ICC/IF: HEK 293T cells. IHC-P: Mouse brain tissues and human kidney tissue. WB: mouse brain lysate
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200.
|WB||1/100 - 1/1000. Predicted molecular weight: 56 kDa.|
FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- 7nAChR antibody
- a7 nicotinic acetylcholine receptor antibody
- acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485) at 1/300 dilution + mouse brain lysate
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/500 dilution
Predicted band size: 56 kDa
Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by Cy3 conjugated secondary Goat Anti-Rabbit IgG. DAPI was used to stain the cell nuclei.
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse brain tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by secondary detection and DAB staining.
Immunofluorescent analysis of HEK293 cells labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/500 dilution, followed by secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated. DAPI staining of nuclei is shown too.
ab216485 has been referenced in 3 publications.
- Xu H et al. Downregulation of a7 nicotinic acetylcholine receptors in peripheral blood monocytes is associated with enhanced inflammation in preeclampsia. BMC Pregnancy Childbirth 19:188 (2019). PubMed: 31138166
- Seoane-Collazo P et al. Central nicotine induces browning through hypothalamic ? opioid receptor. Nat Commun 10:4037 (2019). PubMed: 31492869
- Velazquez R et al. Lifelong choline supplementation ameliorates Alzheimer's disease pathology and associated cognitive deficits by attenuating microglia activation. Aging Cell 18:e13037 (2019). PubMed: 31560162