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  1. Link

    nicotinic-acetylcholine-receptor-alpha-7-antibody-ab216485.pdf

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Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (7)

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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)

Key features and details

  • Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody
    See all Nicotinic Acetylcholine Receptor alpha 7 primary antibodies
  • Description

    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Nicotinic Acetylcholine Receptor alpha 7 aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P36544

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: HEK 293T cells. IHC-P: Mouse brain tissues and human kidney tissue. WB: mouse brain lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • nAch Receptors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Mouse brain tissue lysate - total protein (ab30151)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab216485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

WB (2)
1/100 - 1/1000. Predicted molecular weight: 56 kDa.
ICC/IF (1)
1/500.
Notes
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

WB
1/100 - 1/1000. Predicted molecular weight: 56 kDa.
ICC/IF
1/500.

Target

  • Function

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Target information above from: UniProt accession P36544 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1139 Human
    • Entrez Gene: 11441 Mouse
    • Omim: 118511 Human
    • SwissProt: P36544 Human
    • SwissProt: P49582 Mouse
    • Unigene: 511772 Human
    • Unigene: 113464 Mouse
    • Alternative names

      • 7nAChR antibody
      • a7 nicotinic acetylcholine receptor antibody
      • acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
      • ACHA7_HUMAN antibody
      • AChR antibody
      • Acra7 antibody
      • alpha 7 neuronal nicotinic acetylcholine receptor antibody
      • alpha-7 nicotinic cholinergic receptor subunit antibody
      • alpha7 antibody
      • cholinergic receptor, neuronal nicotinic, alpha polypeptide 7 antibody
      • cholinergic receptor, nicotinic, alpha 7 (neuronal) antibody
      • cholinergic receptor, nicotinic, alpha 7 antibody
      • cholinergic receptor, nicotinic, alpha polypeptide 7 antibody
      • CHRNA7 antibody
      • CHRNA7-2 antibody
      • NACHRA7 antibody
      • neuronal acetylcholine receptor protein, alpha-7 chain antibody
      • Neuronal acetylcholine receptor subunit alpha-7 antibody
      see all

    Images

    • Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
      Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
      Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485) at 1/300 dilution + mouse brain lysate

      Secondary
      Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/500 dilution

      Predicted band size: 56 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)

      Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by Cy3 conjugated secondary Goat Anti-Rabbit IgG. DAPI was used to stain the cell nuclei.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)

      Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse brain tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by secondary detection and DAB staining.

    • Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)
      Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485)

      Immunofluorescent analysis of HEK293 cells labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/500 dilution, followed by secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated. DAPI staining of nuclei is shown too.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab216485? Please let us know so that we can cite the reference in this datasheet.

    ab216485 has been referenced in 7 publications.

    • Wang D  et al. The Availability of the a7-Nicotinic Acetylcholine Receptor in Early Identification of Vulnerable Atherosclerotic Plaques: A Study Using a Novel 18F-Label Radioligand PET. Front Bioeng Biotechnol 9:640037 (2021). PubMed: 33777911
    • Ni H  et al. Nicotine Modulates CTSS (Cathepsin S) Synthesis and Secretion Through Regulating the Autophagy-Lysosomal Machinery in Atherosclerosis. Arterioscler Thromb Vasc Biol 40:2054-2069 (2020). PubMed: 32640907
    • Dong Y  et al. Nicotine Prevents Oxidative Stress-Induced Hippocampal Neuronal Injury Through a7-nAChR/Erk1/2 Signaling Pathway. Front Mol Neurosci 13:557647 (2020). PubMed: 33328880
    • Xu H  et al. Downregulation of a7 nicotinic acetylcholine receptors in peripheral blood monocytes is associated with enhanced inflammation in preeclampsia. BMC Pregnancy Childbirth 19:188 (2019). PubMed: 31138166
    • Seoane-Collazo P  et al. Central nicotine induces browning through hypothalamic ? opioid receptor. Nat Commun 10:4037 (2019). PubMed: 31492869
    • Velazquez R  et al. Lifelong choline supplementation ameliorates Alzheimer's disease pathology and associated cognitive deficits by attenuating microglia activation. Aging Cell 18:e13037 (2019). PubMed: 31560162
    • Wang Q  et al. Dysregulated repair and inflammatory responses by e-cigarette-derived inhaled nicotine and humectant propylene glycol in a sex-dependent manner in mouse lung. FASEB Bioadv 1:609-623 (2019). PubMed: 31825014

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Mouse brain, Rat Brain, HepaRG (human liver))
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    40 µg
    Treatment
    HepaRG treated with siRNA
    Specification
    Mouse brain, Rat Brain, HepaRG (human liver)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    DR. Romain Parent

    Verified customer

    Submitted Aug 04 2021

    Immunohistochemistry free floating abreview for Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 23 2021

    Western blot abreview for Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (Brain lysates)
    Gel Running Conditions
    Reduced Denaturing (12% SDS-PAGE)
    Loading amount
    40 µg
    Specification
    Brain lysates
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    MISS. Elif Karoğlu

    Verified customer

    Submitted Sep 09 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Bend.3 endothelial cell line)
    Permeabilization
    Yes - 0.1% triton
    Specification
    Bend.3 endothelial cell line
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 11 2017

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