Overview

  • Product name

    Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody
    See all Nicotinic Acetylcholine Receptor alpha 7 primary antibodies
  • Description

    Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Nicotinic Acetylcholine Receptor alpha 7 aa 491-502 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    PNFVEAVSKDFA


    Database link: P36544

  • Positive control

    • ICC/IF: HEK 293T cells. IHC-P: Mouse brain tissues and human kidney tissue. WB: mouse brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab216485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

WB 1/100 - 1/1000. Predicted molecular weight: 56 kDa.
ICC/IF 1/500.

Target

  • Function

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 7nAChR antibody
    • a7 nicotinic acetylcholine receptor antibody
    • acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
    • ACHA7_HUMAN antibody
    • AChR antibody
    • Acra7 antibody
    • alpha 7 neuronal nicotinic acetylcholine receptor antibody
    • alpha-7 nicotinic cholinergic receptor subunit antibody
    • alpha7 antibody
    • cholinergic receptor, neuronal nicotinic, alpha polypeptide 7 antibody
    • cholinergic receptor, nicotinic, alpha 7 (neuronal) antibody
    • cholinergic receptor, nicotinic, alpha 7 antibody
    • cholinergic receptor, nicotinic, alpha polypeptide 7 antibody
    • CHRNA7 antibody
    • CHRNA7-2 antibody
    • NACHRA7 antibody
    • neuronal acetylcholine receptor protein, alpha-7 chain antibody
    • Neuronal acetylcholine receptor subunit alpha-7 antibody
    see all

Images

  • Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab216485) at 1/300 dilution + mouse brain lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/500 dilution

    Predicted band size: 56 kDa

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by Cy3 conjugated secondary Goat Anti-Rabbit IgG. DAPI was used to stain the cell nuclei.

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse brain tissue labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/200, followed by secondary detection and DAB staining.

  • Immunofluorescent analysis of HEK293 cells labeling Nicotinic Acetylcholine Receptor alpha 7 using ab216485 at 1/500 dilution, followed by secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated. DAPI staining of nuclei is shown too.

References

ab216485 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bend.3 endothelial cell line)
Permeabilization
Yes - 0.1% triton
Specification
Bend.3 endothelial cell line
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Oct 11 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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