Key features and details
- Goat polyclonal to Nicotinic Acetylcholine Receptor beta 2/CHRNB2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody
See all Nicotinic Acetylcholine Receptor beta 2/CHRNB2 primary antibodies
DescriptionGoat polyclonal to Nicotinic Acetylcholine Receptor beta 2/CHRNB2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Cow, Dog, Pig, Monkey, Gorilla
- Human cerebellum lysate; Human brain, cortex tissue.
This product was previously labelled as Nicotinic Acetylcholine Receptor beta 2
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 57 kDa).
The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
|IHC-P||Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Involvement in diseaseDefects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3) [MIM:605375]. ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-2/CHRNB2 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- Acetylcholine receptor beta 2 neural antibody
- ACHB2_HUMAN antibody
- ACHN antibody
Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab189174) at 1 µg/ml + Human cerebellum lysate (in RIPA buffer) at 35 µg
Predicted band size: 57 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex tissue labeling Nicotinic Acetylcholine Receptor beta 2/CHRNB2 with ab189174 at 3.75 µg/ml.
ab189174 has been referenced in 1 publication.
- Durham E et al. Direct Effects of Nicotine Exposure on Murine Calvaria and Calvarial Cells. Sci Rep 9:3805 (2019). PubMed: 30846819