Product nameAnti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody
See all Nicotinic Acetylcholine Receptor beta 2/CHRNB2 primary antibodies
DescriptionRabbit polyclonal to Nicotinic Acetylcholine Receptor beta 2/CHRNB2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
This product was previously labelled as Nicotinic Acetylcholine Receptor beta 2
FormLyophilised:Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent of whole antiserum
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: PBS, 1% BSA, Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab41174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.|
FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Involvement in diseaseDefects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3) [MIM:605375]. ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-2/CHRNB2 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- Acetylcholine receptor beta 2 neural antibody
- ACHB2_HUMAN antibody
- ACHN antibody
ICC/IF image of ab41174 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41174, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
The band observed at 52 kDa could potentially be a cleaved form of Nicotinic Acetylcholine Receptor beta 2/CHRNB2 due to the presence of a 25 amino acid signal peptide.
ab41174 has not yet been referenced specifically in any publications.