Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174)

Reviews (2)Q&A (1)

Overview

  • Product name

    Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody
    See all Nicotinic Acetylcholine Receptor beta 2/CHRNB2 primary antibodies

  • Description

    Rabbit polyclonal to Nicotinic Acetylcholine Receptor beta 2/CHRNB2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human Nicotinic Acetylcholine Receptor beta 2/CHRNB2 aa 493-502 (C terminal).
    Sequence:

    HSDHSAPSSK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

     This product was previously labelled as Nicotinic Acetylcholine Receptor beta 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab41174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC/IF 1/200.

Target

  • Function

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

  • Involvement in disease

    Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3) [MIM:605375]. ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements.

  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-2/CHRNB2 sub-subfamily.

  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Acetylcholine receptor beta 2 neural antibody
    • ACHB2_HUMAN antibody
    • ACHN antibody
    • AChR antibody
    • Acrb 2 antibody
    • Acrb2 antibody
    • b2 nAchR antibody
    • Cholinergic receptor nicotinic beta 2 antibody
    • Cholinergic receptor nicotinic beta polypeptide 2 antibody
    • Cholinergic receptor nicotinic beta polypeptide 2 neuronal antibody
    • cholinergic receptor, nicotinic, beta 2 (neuronal) antibody
    • Chrnb2 antibody
    • EFNL 3 antibody
    • EFNL3 antibody
    • nAChRB2 antibody
    • Neuronal acetylcholine receptor protein beta 2 chain precursor antibody
    • Neuronal acetylcholine receptor protein subunit beta 2 antibody
    • Neuronal acetylcholine receptor subunit beta-2 antibody
    • Neuronal nicotinic acetylcholine receptor beta 2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174)
    Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174)
    ICC/IF image of ab41174 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41174, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174)
    Western blot - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174)
    All lanes : Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    The band observed at 52 kDa could potentially be a cleaved form of Nicotinic Acetylcholine Receptor beta 2/CHRNB2 due to the presence of a 25 amino acid signal peptide.

References

ab41174 has not yet been referenced specifically in any publications.

Publishing research using ab41174? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 3% Triton X100
Specification
Brain
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 0.5µg/mL · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 15 2015

Western blot abreview for Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody

 Inconclusive
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain sample)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
10 µg
Specification
brain sample
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Mr. M Vandesquille

Verified customer

Submitted Dec 02 2010

Question

I have been incubating in primary antibody at 4 degrees overnight in TBS-T with 1% BSA. I will try the secondary alone condition, but we have used this secondary antibody successfully with other primary antibodies, so I do not think that is the issue. Also, I will try a longer blocking period and more washes. On the datasheet for this antibody there isn't an image of a representative western blot using Abcam ab41174. Could you please send me an image of the band that Abcam has been able to detect by western blot? Also, is there anything else you can suggest for me to try? Or a positive control that you could send me to work with?

Read More
Answer

Thank you for your enquiry. I have added two of the three following articles using this antibody to our datasheet (I have included a datasheet link below for your convenience). The 1997 article is not available via the internet, but is the original source of the antibodies in the literature. In the Nguyen article, there is a nice picture of a western blot. References: 1. Zia, S et al (2000) J Pharmacol Exp Ther, 293: 973-981 2. Nguyen, VT et al (2000) J Dent Res, 79: 939-949 3. Zia S et al (1997) Res Commun Mol Pathol Pharmacol, 97: 243-262 I hope this information helps. Please do not hesitate to contact us if you need anything further.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com