Overview

  • Product name

    Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody
    See all Nicotinic Acetylcholine Receptor epsilon/CHRNE primary antibodies
  • Description

    Rabbit polyclonal to Nicotinic Acetylcholine Receptor epsilon/CHRNE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human Nicotinic Acetylcholine Receptor epsilon/CHRNE.

  • General notes

     This product was previously labelled as Nicotinic Acetylcholine Receptor epsilon

     

Properties

Applications

Our Abpromise guarantee covers the use of ab65180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/5000. Predicted molecular weight: 55 kDa.

Target

  • Function

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in disease

    The muscle AChR is the major target antigen in the autoimmune disease myasthenia gravis. Myasthenia gravis is characterized by sporadic muscular fatigability and weakness, occurring chiefly in muscles innervated by cranial nerves, and characteristically improved by cholinesterase-inhibiting drugs.
    Myasthenic syndrome, congenital, 4A, slow-channel
    Myasthenic syndrome, congenital, 4B, fast-channel
    Myasthenic syndrome, congenital, 4C, associated with acetylcholine receptor deficiency
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Epsilon/CHRNE sub-subfamily.
  • Cellular localization

    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acetylcholine receptor subunit epsilon antibody
    • ACHE_HUMAN antibody
    • AChR antibody
    • AchR epsilon subunit antibody
    • ACHRE antibody
    • Cholinergic receptor, nicotinic, epsilon polypeptide antibody
    • Chrne antibody
    • CMS1D antibody
    • CMS1E antibody
    • CMS2A antibody
    • FCCMS antibody
    • SCCMS antibody
    see all

References

This product has been referenced in:

  • Saeed S  et al. Conformational co-dependence between Plasmodium berghei LCCL proteins promotes complex formation and stability. Mol Biochem Parasitol 185:170-3 (2012). Read more (PubMed: 22877575) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (primary muscle cells)
Permeabilization
Yes - triton
Specification
primary muscle cells
Blocking step
BSA + goat serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde

Mrs. Susie Barbeau

Verified customer

Submitted Apr 19 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (tibialis muscle)
Gel Running Conditions
Non-reduced Denaturing (10% gel BioRad, Running Buffer BioRad.)
Loading amount
25 µg
Specification
tibialis muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Ms. Lisa Marienhoff

Verified customer

Submitted Dec 03 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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