Product nameAnti-Nidogen 2/NID-2 antibody
See all Nidogen 2/NID-2 primary antibodies
DescriptionRabbit polyclonal to Nidogen 2/NID-2
Tested applicationsSuitable for: WB, ELISA, IHC-Fr, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse
Recombinant full length protein corresponding to Mouse Nidogen 2/NID-2.
Previously labelled as Nidogen 2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Our Abpromise guarantee covers the use of ab14513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 153 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 23211522
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/200. PubMed: 23409002|
FunctionCell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
Tissue specificityHeart, placenta and bone. Less in pancreas, kidney and skeletal muscle.
Sequence similaritiesContains 5 EGF-like domains.
Contains 5 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 2 thyroglobulin type-1 domains.
modificationsHighly N- and O-glycosylated.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- NID 2 antibody
- NID-2 antibody
- Nid2 antibody
All lanes : Anti-Nidogen 2/NID-2 antibody (ab14513) at 1/400 dilution
Lane 1 : Recombinant Nidogen 2 at 0.005 µg
Lane 2 : Recombinant Nidogen 1
Lane 3 : Protein extract of mouse femoral heads. The femoral head extract was prepared by incubating 3 mouse femoral heads (total weight 20 mg) in 200 µl buffer (0.05 M Tris; 0.15 M NaCl; 10 mM EDTA; 6 M Urea, pH 7.4) overnight. at 20 µl
Performed under reducing conditions.
Predicted band size: 153 kDa
This product has been referenced in:
- Nordquist E et al. Postnatal and Adult Aortic Heart Valves Have Distinctive Transcriptional Profiles Associated With Valve Tissue Growth and Maintenance Respectively. Front Cardiovasc Med 5:30 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29740591) »
- Murphy PA et al. Tumor angiogenesis in the absence of fibronectin or its cognate integrin receptors. PLoS One 10:e0120872 (2015). Read more (PubMed: 25807551) »