Key features and details
- Rabbit polyclonal to Nidogen 2/NID-2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nidogen 2/NID-2 antibody
See all Nidogen 2/NID-2 primary antibodies
DescriptionRabbit polyclonal to Nidogen 2/NID-2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment (His-T7-tag) corresponding to Human Nidogen 2/NID-2 aa 31-289. (Expressed in E.coli).
LHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTN GIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSP AVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELN TFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRG EADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVR PAAVGDLSA
Database link: Q14112
- IHC-P: Human breast cancer and stomach tissues. WB: Human lung lysate; Recombinant human Nidogen 2/NID-2 protein.
Previously labelled as Nidogen 2.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232883 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 151 kDa.|
FunctionCell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
Tissue specificityHeart, placenta and bone. Less in pancreas, kidney and skeletal muscle.
Sequence similaritiesContains 5 EGF-like domains.
Contains 5 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 2 thyroglobulin type-1 domains.
modificationsHighly N- and O-glycosylated.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- NID 2 antibody
- NID-2 antibody
- Nid2 antibody
Anti-Nidogen 2/NID-2 antibody (ab232883) at 2 µg/ml + Human lung lysate
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 151 kDa
Paraffin-embedded human breast cancer tissue stained for Nidogen 2/NID-2 using ab232883 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human stomach tissue stained for Nidogen 2/NID-2 using ab232883 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Nidogen 2/NID-2 antibody (ab232883) at 2 µg/ml + Recombinant human Nidogen 2/NID-2 protein
Predicted band size: 151 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232883 has been referenced in 1 publication.
- Wang J et al. Silencing NID2 by DNA Hypermethylation Promotes Lung Cancer. Pathol Oncol Res N/A:N/A (2019). PubMed: 30826972