Overview

  • Product name

    Anti-Nidogen 2/NID-2 antibody [EPR8697]
    See all Nidogen 2/NID-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8697] to Nidogen 2/NID-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human Nidogen 2/NID-2 aa 1-100 (N terminal).
    Database link: Q14112

  • Positive control

    • Human fetal kidney, BxPC-3, fetal heart, and fetal lung lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as Nidogen 2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 151 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
    • Tissue specificity

      Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle.
    • Sequence similarities

      Contains 5 EGF-like domains.
      Contains 5 LDL-receptor class B repeats.
      Contains 1 NIDO domain.
      Contains 1 nidogen G2 beta-barrel domain.
      Contains 2 thyroglobulin type-1 domains.
    • Post-translational
      modifications

      Highly N- and O-glycosylated.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • NID 2 antibody
      • NID-2 antibody
      • Nid2 antibody
      • NID2_HUMAN antibody
      • Nidogen-2 antibody
      • Nidogen2 antibody
      • Osteonidogen antibody
      see all

    Images

    • All lanes : Anti-Nidogen 2/NID-2 antibody [EPR8697] (ab131279) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : BxPC-3 lysate
      Lane 3 : Human fetal heart lysate
      Lane 4 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 151 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Bartoschek M  et al. Spatially and functionally distinct subclasses of breast cancer-associated fibroblasts revealed by single cell RNA sequencing. Nat Commun 9:5150 (2018). Read more (PubMed: 30514914) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131279.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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