Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing (NuPAGE™ Novex™ 8% Bis-Tris Midi Protein Gels, 26-well)
Loading amount
30 µg
Specification
liver
Blocking step
Rockland Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: DPBS/blocking buffer(50/50)+ 0,1%Tween20
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye® 800CW Donkey anti-Rabbit IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Rabbit
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
fluo
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 180 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Eric DESSAUD
Verified customer
Submitted Dec 05 2023