Recombinant
RabMAb

Recombinant Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)

Overview

  • Product name

    Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717]
    See all Niemann Pick C1 Like 1/NPC1L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5717] to Niemann Pick C1 Like 1/NPC1L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human Niemann Pick C1 Like 1/NPC1L1 aa 1300 to the C-terminus.

  • Positive control

    • Human fetal liver and HeLa lysates; Human liver tissue.
  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Niemann Pick C1 Like 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism.
    • Tissue specificity

      Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle.
    • Sequence similarities

      Belongs to the patched family.
      Contains 1 SSD (sterol-sensing) domain.
    • Post-translational
      modifications

      Highly glycosylated.
    • Cellular localization

      Apical cell membrane. Cell membrane. Cytoplasmic vesicle membrane. Subfractionation of brush border membranes from proximal enterocytes suggests considerable association with the apical membrane fraction. Exists as a predominantly cell surface membrane expressed protein (By similarity). According to PubMed:15671032, localizes in a subcellular vesicular compartment rich in RAB5.
    • Information by UniProt
    • Database links

    • Alternative names

      • Niemann Pick C1 like protein 1 antibody
      • Niemann Pick disease type C1 gene like 1 antibody
      • Niemann-Pick C1-like protein 1 antibody
      • NPC1 LIKE 1 antibody
      • NPC1 Niemann Pick disease type C1 gene like 1 antibody
      • NPC1L1 antibody
      • NPC1L1 splice variant antibody
      • NPCL1_HUMAN antibody
      • Truncated Niemann Pick C1 like protein 1 antibody
      see all

    Images

    • Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/1000 dilution (purified) + HeLa whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 149 kDa
      Observed band size: 149 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/10000 dilution (purified) + Human fetal liver lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 149 kDa
      Observed band size: 149 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal liver lysates
      Lane 2 : HeLa lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 149 kDa

    References

    This product has been referenced in:

    • Yang F  et al. Fatty acids modulate the expression levels of key proteins for cholesterol absorption in Caco-2 monolayer. Lipids Health Dis 17:32 (2018). Read more (PubMed: 29463265) »
    • Yang F  et al. Egg-Yolk Sphingomyelin and Phosphatidylcholine Attenuate Cholesterol Absorption in Caco-2 Cells. Lipids 53:217-233 (2018). Read more (PubMed: 29569242) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T cells over expressing human NPC1L1)
    Loading amount
    100 µg
    Specification
    HEK293T cells over expressing human NPC1L1
    Gel Running Conditions
    Reduced Denaturing (7.5% Tris-Glycine gel)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Feb 06 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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