HEK293T cells over expressing human NPC1L1-ACP tagged protein
HEK293T cells over expressing mouse NPC1-GFP tagged protein
Human NPC1L1-ACP tagged protein (predicted MW of 160 kDa) and mouse NPC1-GFP tagged protein (predicted MW of 175 kDa) were over expressed in HEK293T cells for 48 hr and lysed in a buffer containing 50mM Tris-HCl pH 7.4, 150 mM NaCl, 5mM MgCl2, 1mM EDTA, 1% Triton X-100, 10% glycerol and protease inhibitors. 100 µg of total cell lysate (with Laemmli buffer) was heated at 50ºC for 25 min. and SDS-PAGE followed by western blotting was performed.
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Submitted Feb 06 2013
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