Recombinant
RabMAb

Recombinant Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (HRP) (ab201773)

Overview

  • Product name

    Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (HRP)
    See all Niemann Pick C1 Like 1/NPC1L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5717] to Niemann Pick C1 Like 1/NPC1L1 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human Niemann Pick C1 Like 1/NPC1L1 aa 1300 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal liver tissue lysate. IHC-P: FFPE normal human liver tissue sections.
  • General notes

     

     

     This product was previously labelled as Niemann Pick C1 Like 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).

Target

  • Function

    Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism.
  • Tissue specificity

    Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle.
  • Sequence similarities

    Belongs to the patched family.
    Contains 1 SSD (sterol-sensing) domain.
  • Post-translational
    modifications

    Highly glycosylated.
  • Cellular localization

    Apical cell membrane. Cell membrane. Cytoplasmic vesicle membrane. Subfractionation of brush border membranes from proximal enterocytes suggests considerable association with the apical membrane fraction. Exists as a predominantly cell surface membrane expressed protein (By similarity). According to PubMed:15671032, localizes in a subcellular vesicular compartment rich in RAB5.
  • Information by UniProt
  • Database links

  • Alternative names

    • Niemann Pick C1 like protein 1 antibody
    • Niemann Pick disease type C1 gene like 1 antibody
    • Niemann-Pick C1-like protein 1 antibody
    • NPC1 LIKE 1 antibody
    • NPC1 Niemann Pick disease type C1 gene like 1 antibody
    • NPC1L1 antibody
    • NPC1L1 splice variant antibody
    • NPCL1_HUMAN antibody
    • Truncated Niemann Pick C1 like protein 1 antibody
    see all

Images

  • Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (HRP) (ab201773) at 1/5000 dilution + Liver (Human) Tissue Lysate - fetal normal tissue at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 149 kDa
    Observed band size: 149 kDa


    Exposure time: 12 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201773 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

This product has been referenced in:

  • Chen KJ  et al. The prognostic value of Niemann-Pick C1-like protein 1 and Niemann-Pick disease type C2 in hepatocellular carcinoma. J Cancer 9:556-563 (2018). Read more (PubMed: 29483961) »
See 1 Publication for this product

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