Product nameAnti-Niemann Pick C2 antibody
See all Niemann Pick C2 primary antibodies
DescriptionRabbit polyclonal to Niemann Pick C2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human Niemann Pick C2.
Database link: P61916
- 293T, M21, mouse lung, mouse spleen and mouse liver cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab186829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 17 kDa.|
FunctionMay be involved in the regulation of the lipid composition of sperm membranes during the maturation in the epididymis.
Involvement in diseaseDefects in NPC2 are the cause of Niemann-Pick disease type C2 (NPDC2) [MIM:607625]. A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C2 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood.
Sequence similaritiesBelongs to the NPC2 family.
- Information by UniProt
- EDDM1 antibody
- Epididymal protein 1 antibody
- Epididymal secretory protein antibody
All lanes : Anti-Niemann Pick C2 antibody (ab186829) at 1/500 dilution
Lane 1 : 293T cell extract
Lane 2 : M21 cell extract
Lane 3 : Mouse lung cell extract
Lane 4 : Mouse spleen cell extract
Lane 5 : Mouse liver cell extract
Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Niemann Pick C2 with ab186829 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling Niemann Pick C2 with ab186829 at 1/200. Magnification: 200x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Niemann Pick C2 with ab186829 at 1/200. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186829. Blue DAPI for nuclear staining.
ab186829 has been referenced in 1 publication.
- Wichit S et al. Imipramine Inhibits Chikungunya Virus Replication in Human Skin Fibroblasts through Interference with Intracellular Cholesterol Trafficking. Sci Rep 7:3145 (2017). PubMed: 28600536