Key features and details
- Mouse monoclonal [CL2237] to NIFK
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-NIFK antibody [CL2237]
See all NIFK primary antibodies
DescriptionMouse monoclonal [CL2237] to NIFK
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
EpitopeBinds to an epitope located within the peptidesequence IDYDFPSLILQKTES as determined by overlapping synthetic peptides.
- IHC-P: Human skin, small intestine, pancreas, kidney, liver and fallopian tube tissues. WB: U-251 MG cell lysate. ICC/IF: U-2 OS cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab244216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 2 - 10 µg/ml.
Fixation: PFA/Triton X-100
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.|
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsSequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
Cellular localizationNucleus > nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
- Information by UniProt
- hNIFK antibody
- MK67I_HUMAN antibody
- MKI67 FHA Domain Interacting Nucleolar Phosphoprotein antibody
PFA-fixed, Triton X-100 permeabilized U-2 OS cells stained for MK167 (Green) using ab244216 at 4 μg/ml in ICC/IF.
Microtublues are stained red.
Paraffin-embedded human fallopian tube tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreas tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Paraffin-embedded human skin tissue stained for NIFK using ab244216 at 1/2500 dilution in immunohistochemical analysis.
Anti-NIFK antibody [CL2237] (ab244216) at 1 µg/ml + U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 34 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244216 has not yet been referenced specifically in any publications.