Overview

  • Product name
    NIH OVCAR-3 nuclear extract lysate
  • General notes
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    The cell line NIH:OVCAR-3 has been established from the malignant ascites of a patient with progressive adenocarcinoma of the ovary after combination chemotherapy with cyclophosphamide, Adriamycin, and cisplatin. OVCAR-3 grows as a cobblestone-like monolayer with foci of multilayering, is tumorigenic in athymic mice, clones in agarose, and has an abnormal karyotype which includes a homogeneous staining region and a double minute chromosome. The cultured cells have an epithelial morphology and contain cytoplasmic androgen- and estrogen-binding macromolecules with the specificity of the respective steroid hormone receptors. OVCAR-3 may be of use for investigations as to the significance of androgens and estrogens and the mechanisms of cytotoxic drug resistance in ovarian cancer.

References

ab14879 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Q&A

Question
Answer

The nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Hence, you will need to add Laemmli buffer and boil it before you run a gel and do a Western.

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Answer

Merci pour ces précisions.

J'aimerais vous informer que nous n'avons aucun accord commercial avec LuBioScience et que ce revendeur n'est pas en droit de distribuer nos produits. La garantie Abpromise d'Abcam ne s'applique que sur les produits commandés directement chez Abcam ou via un distributeur agréé (https://www.abcam.com/abpromise).

Je m'engage cependant à faire exception pour le cas présent et accepte de vous envoyer gratuitement une unité de ab14879 directement à votre laboratoire comme convenu précédemment. Le numéro de la commande est ******.

Pourriez-vous, s'il vous plait, placer vos futures commandes directement chez Abcam et, si possible, informer vos collègues que la société LuBioScience n'est pas autorisée à revendre les produits Abcam.

N'hésitez pas à m'informer des nouveaux résultats obtenus avec l'anticorps ab134093 et le lysat ab14879.

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Answer

Thank you for your enquiry. This lysate contains only PMSF as a protease inhibitor. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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