NIH/3T3 cytoplasmic extract lysate (ab14873)

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Overview

  • Product name

    NIH/3T3 cytoplasmic extract lysate
    See all NIH 3T3 lysates

  • Description

    NIH/3T3 cytoplasmic extract

  • General notes

    Cell line: NIH/3T3 (Mouse embryonic fibroblast).
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.90
    Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
  • Concentration information loading...

  • Research areas

  • Background

    NIH 3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is therefore used for DNA transfection studies

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab14873? Please let us know so that we can cite the reference in this datasheet.

ab14873 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Customer reported only receiving about 1 ul in her vial of ab14873 from order# 131115.

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Answer

Thank you for your phone call and I apologize for the shortage of ab14873 that you received. The free of charge replacement vial is being sent to your attention on order reference # 133497. The item is currently out of stock but you will receive email confirmations regarding shipping. Please contact us again if you have any additional questions or concerns. Have a great weekend!

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