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I am interested in purchasing a fibroblast whole cell lysate product from you company to test a new procollagen probe my lab developed.
I noticed that you sell several kinds of fibroblast lysate products:ab7179, ab3960, ab3874, ab7901...
I wanna know if these products have a high procollagen (˜150KDa) content. Have you tested that? Or do you know if there have been any existing reports in which these products have been used for procollagen detection? This information is important for me to make the decision.
Asked on Apr 10 2012
Thank you for contacting Abcam and for your interest in our whole cell lysates.
Regarding these products, they are not tested for protein composition. They are only tested for protein concentration and confirmed to not be degraded.
If you are interested in determining which lysates will contain the protein that you need, I would recommend a search of the literature. Unfortunately, we are not experts in this field and would not want to recommend a product that is not suitable for your experiments.
I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.
Answered on Apr 10 2012