Product nameAnti-Ninein antibody - Centrosome Marker
See all Ninein primary antibodies
DescriptionRabbit polyclonal to Ninein - Centrosome Marker
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Fusion protein containing amino acids 32-506 of murine Ninein
- This antibody gave a positive signal in the following cell lines: HeLa; MCF7.
The centrosomal protein ninein has been identified as a microtubule minus end capping and centriole anchoring protein, but its true physiological function remains to be determined. At mitosis, ninein has been observed in association with the mitotic spindle as well as at the centrosomes.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
Primary antibody notesThe centrosomal protein ninein has been identified as a microtubule minus end capping and centriole anchoring protein, but its true physiological function remains to be determined. At mitosis, ninein has been observed in association with the mitotic spindle as well as at the centrosomes.
Our Abpromise guarantee covers the use of ab4447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 28469279|
|WB||1/500. Detects a band of approximately 249 kDa (predicted molecular weight: 249 kDa).|
- Information by UniProt
- Centrosome Marker antibody
- Glycogen synthase kinase 3 beta interacting protein antibody
- GSK3B interacting protein antibody
IF using ab4447 to detect Ninein in MCF7 cells (human).
The left hand panels are DAPI stained nuclei. The right hand panels are the same nuclei stained for Ninein.
ab4447 was used at 1/1000.
Ninein shows nuclear and centrosome staining, which is expected from the literature.
ICC/IF image of ab4447 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4447, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab4447 shows the characteristic nuclear and centrosome staining for Ninein.
This product has been referenced in:
- Magescas J et al. Spindle pole cohesion requires glycosylation-mediated localization of NuMA. Sci Rep 7:1474 (2017). IHC-P ; Mouse . Read more (PubMed: 28469279) »
- Jao LE et al. A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies. Mol Biol Cell 28:120-127 (2017). Read more (PubMed: 28035044) »