Product nameNinhydrin, Amine detection reagent
DescriptionAmine detection reagent.
- 1,2,3-Indantrione monohydrate
Ninhydrin reacts with ammonia and primary and secondary amines producing a blue or purple reaction product (diketohydrindylidene-diketohydrindamine). Commonly used for the development of fingermarks on porous surfaces. Also useful in the analysis of free amino groups in proteins, peptides and amino acids. May be useful for detecting ninhydrin-reactive nitrogens in soil for forensic applications.
Storage instructionsStore at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Song DF et al. Thermal development of latent fingermarks on porous surfaces--further observations and refinements. Forensic Sci Int 204:97-110 (2011). Read more (PubMed: 20554406) »
- Carter DO et al. Using ninhydrin to detect gravesoil. J Forensic Sci 53:397-400 (2008). Read more (PubMed: 18366573) »
- Sheng S et al. A specific quantitative colorimetric assay for L-asparagine. Anal Biochem 211:242-9 (1993). Read more (PubMed: 8100404) »
- Moore S Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction. J Biol Chem 243:6281-3 (1968). Read more (PubMed: 5723468) »
- MOORE S & STEIN WH Photometric ninhydrin method for use in the chromatography of amino acids. J Biol Chem 176:367-88 (1948). Read more (PubMed: 18886175) »