Key features and details
- Rabbit polyclonal to NINL
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NINL antibody
See all NINL primary antibodies
DescriptionRabbit polyclonal to NINL
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to amino acids 682-787 of Human NINL.
- Human cerebellum tissue. ICC/IF: U2-OS cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/0.25 - 1/2.
Fixation/Permeabilization: PFA/Triton X-100
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis.
Tissue specificityExpressed in KYSE150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina.
Sequence similaritiesContains 4 EF-hand domains.
DomainThe KEN and D (destructive) boxes are required for the cell cycle-controlled NINL degradation by the APC/C pathway.
modificationsPhosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin.
Ubiquitinated by the APC/C complex leading to its degradation.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm. In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm.
- Information by UniProt
- 4930519N13Rik antibody
- dJ691N24.1 antibody
- Gm1004, antibody
ab121101 at 1/100 dilution staining NINL in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.
Note: moderate cytoplasmic positivity in purkinje cells.
Immunocytochemistry/ Immunofluorescence analysis of U-2 OS labeling NINL with ab121110. The image shows localization to cytosol & cytokinetic bridge.
Fixation/Permeabilization: PFA/Triton X-100
ab121110 has been referenced in 1 publication.
- Yin L et al. Epidermal gene expression and ethnic pigmentation variations among individuals of Asian, European and African ancestry. Exp Dermatol 23:731-5 (2014). IHC-P ; Human . PubMed: 25055985