Key features and details
- Rabbit polyclonal to NINL
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NINL antibody
See all NINL primary antibodies
DescriptionRabbit polyclonal to NINL
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human NINL aa 1-190.
MDEEENHYVSQLREVYSSCDTTGTGFLDRQELTQLCLKLHLEQQLPVLLQ TLLGNDHFARVNFEEFKEGFVAVLSSNAGVRPSDEDSSSLESAASSAIPP KYVNGSKWYGRRSRPELCDAATEARRVPEQQTQASLKSHLWRSASLESVE SPKSDEEAESTKEAQNELFEAQGQLQTWDSEDFGSPQKSC
Database link: Q9Y2I6
- IHC-P: Human brain and adrenal gland tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab234784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
FunctionInvolved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis.
Tissue specificityExpressed in KYSE150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina.
Sequence similaritiesContains 4 EF-hand domains.
DomainThe KEN and D (destructive) boxes are required for the cell cycle-controlled NINL degradation by the APC/C pathway.
modificationsPhosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin.
Ubiquitinated by the APC/C complex leading to its degradation.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm. In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm.
- Information by UniProt
- 4930519N13Rik antibody
- dJ691N24.1 antibody
- Gm1004, antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234784 has not yet been referenced specifically in any publications.