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Rabbit polyclonal to NIP7
Predicted to work with:
Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide, corresponding to a region within amino acids 130-180 of Human NIP7 (NP_057185.1).
HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
Use at 2-10 µg/mg of lysate.
Required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly.
Expressed in hematopoietic stem/progenitor cells.
Belongs to the NIP7 family.
Contains 1 PUA domain.
Nucleus > nucleolus.
Information by UniProt
60S ribosome subunit biogenesis protein NIP7 homolog antibody
CGI 37 antibody
Western blot - Anti-NIP7 antibody (ab168814)
All lanes : Anti-NIP7 antibody (ab168814) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate Lane 2 : 293T whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : TCMK-1 whole cell lysate Lane 5 : NIH3T3 whole cell lysate Lysates/proteins at 50 µg per lane. Developed using the ECL technique. Predicted band size: 20 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-NIP7 antibody (ab168814)
Detection of NIP7 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168814 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 shows control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"