Key features and details
- Rabbit polyclonal to NIPA
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NIPA antibody
See all NIPA primary antibodies
DescriptionRabbit polyclonal to NIPA
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176847 was affinity purified using an epitope specific to NIPA immobilized on solid support.
Our Abpromise guarantee covers the use of ab176847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 55 kDa.|
FunctionEssential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.
Tissue specificityWidely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 C3HC-type zinc finger.
Developmental stageWeakly expressed in G0/G1 phases, abundant during S and G2/M phases, and strongly decreases thereafter.
DomainThe F-box-like region is required for the interaction with SKP1.
modificationsPhosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-354 phosphorylation, a major site during the course of cell-cycle-dedendent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry.
- Information by UniProt
- hNIPA antibody
- NIPA_HUMAN antibody
- Nuclear interacting partner of ALK antibody
All lanes : Anti-NIPA antibody (ab176847) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 10 seconds
Detection of NIPA in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176847 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176847 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab176847 has not yet been referenced specifically in any publications.