Anti-NIPA1 antibody (ab121744)

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Overview

  • Product name

    Anti-NIPA1 antibody
    See all NIPA1 primary antibodies

  • Description

    Rabbit polyclonal to NIPA1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant Protein Epitope Signature Tag, corresponding to amino acids 183-226 (HGPTNIMVYI SICSLLGSFT VPSTKGIGLA AQDILHNNPS SQRA) of Human NIPA1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human urinary bladder tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).

  • Tissue specificity

    Widely expressed with highest levels in neuronal tissues.

  • Involvement in disease

    Defects in NIPA1 are the cause of spastic paraplegia autosomal dominant type 6 (SPG6) [MIM:600363]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.

  • Sequence similarities

    Belongs to the NIPA family.

  • Cellular localization

    Cell membrane. Early endosome. Recruited to the cell membrane in response to low extracellular magnesium.
  • Information by UniProt

  • Database links

    • Alternative names

      • FSP 3 antibody
      • FSP3 antibody
      • Magnesium transporter NIPA1 antibody
      • MGC102724 antibody
      • MGC35570 antibody
      • NIPA 1 antibody
      • NIPA1 antibody
      • NIPA1_HUMAN antibody
      • Non imprinted in Prader Willi/Angelman syndrome region protein 1 antibody
      • Non-imprinted in Prader-Willi/Angelman syndrome region protein 1 antibody
      • Spastic paraplegia 6 (autosomal dominant) antibody
      • Spastic paraplegia 6 protein antibody
      • SPG 6 antibody
      • SPG6 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-NIPA1 antibody (ab121744)
      Immunocytochemistry/ Immunofluorescence - Anti-NIPA1 antibody (ab121744)
      Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121744 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIPA1 antibody (ab121744)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIPA1 antibody (ab121744)
      ab121744, at a 1/10 dilution, staining NIPA1 in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.

    References

    ab121744 has not yet been referenced specifically in any publications.

    Publishing research using ab121744? Please let us know so that we can cite the reference in this datasheet.

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