Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActs as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).
Tissue specificityWidely expressed with highest levels in neuronal tissues.
Involvement in diseaseDefects in NIPA1 are the cause of spastic paraplegia autosomal dominant type 6 (SPG6) [MIM:600363]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Sequence similaritiesBelongs to the NIPA family.
Cellular localizationCell membrane. Early endosome. Recruited to the cell membrane in response to low extracellular magnesium.
- Information by UniProt
- FSP 3 antibody
- FSP3 antibody
- Magnesium transporter NIPA1 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121744 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121744, at a 1/10 dilution, staining NIPA1 in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
ab121744 has not yet been referenced specifically in any publications.