Anti-NIPBL antibody (ab225908)
Key features and details
- Rabbit polyclonal to NIPBL
- Suitable for: IHC-P, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-NIPBL antibody
See all NIPBL primary antibodies -
Description
Rabbit polyclonal to NIPBL -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human NIPBL aa 2523-2697.
Database link: Q6KC79 -
Positive control
- IHC-P: Human liver tissue. WB: Recombinant human NIPBL protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/5000. Predicted molecular weight: 316 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/5000. Predicted molecular weight: 316 kDa. |
Target
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Function
Probably plays a structural role in chromatin. Involved in sister chromatid cohesion, possibly by interacting with the cohesin complex. -
Tissue specificity
Widely expressed. Highly expressed in heart, skeletal muscle, fetal and adult liver, fetal and adult kidney. Expressed at intermediates level in thymus, placenta, peripheral leukocyte and small intestine. Weakly or not expressed in brain, colon, spleen and lung. -
Involvement in disease
Cornelia de Lange syndrome 1 -
Sequence similarities
Belongs to the SCC2/Nipped-B family.
Contains 5 HEAT repeats. -
Developmental stage
In embryos, it is expressed in developing limbs and later in cartilage primordia of the ulna and of various hand bones. Sites of craniofacial expression include the cartilage primordium of the basioccipital and basisphenoid skull bones and elsewhere in the head and face, including a region encompassing the mesenchyme adjacent to the cochlear canal. Also expressed in the spinal column, notochord and surface ectoderm sclerotome and what seem to be migrating myoblasts. Expressed in the developing heart in the atrial and ventricular myocardium and in the ventricular tubeculae but absent in the endocardial cushions. Also expressed in the developing esophagus, trachea and midgut loops, in the bronchi of the lung and in the tubules of the metanephros. Expression in organs and tissues not typically affected in CDL (e.g. the developing trachea, bronchi, esophagus, heart and kidney) may reflect a bias towards underreporting of more subtle aspects of the phenotype or problems that typically present later in life. Expressed in the mesenchyme surrounding the cochlear canal possibly reflecting the hearing impairment commonly found. Weakly or not expressed in embryonic brain. -
Domain
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 25836 Human
- Omim: 608667 Human
- SwissProt: Q6KC79 Human
- Unigene: 481927 Human
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Form
There are 3 isoforms produced by alternative splicing. Isoform 1 also known as: A; IDN3-A. Isoform 2 also known as: B; IDN3-B. -
Alternative names
- CDLS antibody
- Colon tumor susceptibility 2 antibody
- Delangin antibody
see all
Images
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Paraffin-embedded human liver tissue stained for NIPBL using ab225908 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-NIPBL antibody (ab225908) at 1/500 dilution
Lane 1 : Recombinant human NIPBL protein at 0.1 µg
Lane 2 : Recombinant human NIPBL protein at 0.05 µg
Lane 3 : Recombinant human NIPBL protein at 0.025 µg
Lane 4 : Recombinant human NIPBL protein at 0.0125 µg
Predicted band size: 316 kDaThis antibody has only been tested in WB using the recombinant protein, we have no data for endogenous protein.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab225908 has been referenced in 1 publication.
- Zhang J et al. Long non-coding RNA NEAT1 regulates glioma cell proliferation and apoptosis by competitively binding to microRNA-324-5p and upregulating KCTD20 expression. Oncol Rep 46:N/A (2021). PubMed: 33982764