Key features and details
- Rabbit polyclonal to NIPBL
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NIPBL antibody
See all NIPBL primary antibodies
DescriptionRabbit polyclonal to NIPBL
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human NIPBL aa 2523-2697.
Database link: Q6KC79
- IHC-P: Human liver tissue. WB: Recombinant human NIPBL protein.
This product was previously labelled as IDN3
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab225908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 316 kDa.|
FunctionProbably plays a structural role in chromatin. Involved in sister chromatid cohesion, possibly by interacting with the cohesin complex.
Tissue specificityWidely expressed. Highly expressed in heart, skeletal muscle, fetal and adult liver, fetal and adult kidney. Expressed at intermediates level in thymus, placenta, peripheral leukocyte and small intestine. Weakly or not expressed in brain, colon, spleen and lung.
Involvement in diseaseCornelia de Lange syndrome 1
Sequence similaritiesBelongs to the SCC2/Nipped-B family.
Contains 5 HEAT repeats.
Developmental stageIn embryos, it is expressed in developing limbs and later in cartilage primordia of the ulna and of various hand bones. Sites of craniofacial expression include the cartilage primordium of the basioccipital and basisphenoid skull bones and elsewhere in the head and face, including a region encompassing the mesenchyme adjacent to the cochlear canal. Also expressed in the spinal column, notochord and surface ectoderm sclerotome and what seem to be migrating myoblasts. Expressed in the developing heart in the atrial and ventricular myocardium and in the ventricular tubeculae but absent in the endocardial cushions. Also expressed in the developing esophagus, trachea and midgut loops, in the bronchi of the lung and in the tubules of the metanephros. Expression in organs and tissues not typically affected in CDL (e.g. the developing trachea, bronchi, esophagus, heart and kidney) may reflect a bias towards underreporting of more subtle aspects of the phenotype or problems that typically present later in life. Expressed in the mesenchyme surrounding the cochlear canal possibly reflecting the hearing impairment commonly found. Weakly or not expressed in embryonic brain.
DomainContains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
- Information by UniProt
FormThere are 3 isoforms produced by alternative splicing. Isoform 1 also known as: A; IDN3-A. Isoform 2 also known as: B; IDN3-B.
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Paraffin-embedded human liver tissue stained for NIPBL using ab225908 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-NIPBL antibody (ab225908) at 1/500 dilution
Lane 1 : Recombinant human NIPBL protein at 0.1 µg
Lane 2 : Recombinant human NIPBL protein at 0.05 µg
Lane 3 : Recombinant human NIPBL protein at 0.025 µg
Lane 4 : Recombinant human NIPBL protein at 0.0125 µg
Predicted band size: 316 kDa
This antibody has only been tested in WB using the recombinant protein, we have no data for endogenous protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225908 has not yet been referenced specifically in any publications.