Overview

  • Product name

  • Description

    Goat polyclonal to NIPP1
  • Host species

    Goat
  • Specificity

    ab129286 is expected to recognise all three Human isoforms of this protein.
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish, a wide range of other species
  • Immunogen

    Synthetic peptide:

    EAWPGKKPTPSLLI

    with a Cysteine residue linker, corresponding to C terminal amino acids 338-351 of Human NIPP1 (NP_002704.1; NP_054829.2; NP_612568.1).

  • Positive control

    • Human Thymus tissue; H460 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab129286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 38 kDa.
IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/32000.

Target

  • Function

    Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
    Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.
  • Sequence similarities

    Contains 1 FHA domain.
  • Domain

    Has a basic N- and C-terminal and an acidic central domain.
  • Post-translational
    modifications

    May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
  • Cellular localization

    Nucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator of RNA decay antibody
    • ARD 1 antibody
    • ARD-1 antibody
    • ARD1 antibody
    • Homolog of E.coli RNase E antibody
    • NIPP 1 antibody
    • NIPP-1 antibody
    • NIPP1 antibody
    • Nuclear inhibitor of protein phosphatase 1 antibody
    • nuclear inhibitor of protein phosphatase-1 alpha antibody
    • Nuclear subunit of PP1 antibody
    • PP1R8_HUMAN antibody
    • PPP1R8 antibody
    • PRO2047 antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 8 antibody
    • Protein phosphatase 1 regulatory subunit 8 antibody
    • RNase E antibody
    • RNase E, E. coli, homolog of antibody
    see all

Images

  • Anti-NIPP1 antibody (ab129286) at 2 µg/ml + H460 lysate (in RIPA buffer) at 30 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa

  • ab129286, at 3.75 µg/ml, staining NIPP1 in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry.

References

ab129286 has not yet been referenced specifically in any publications.

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