Overview

  • Product name

  • Description

    Goat polyclonal to NIPP1
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human NIPP1 aa 114-127 (C terminal).
    Sequence:

    C-EAWPGKKPTPSLLI


    (Peptide available as ab23096)

  • Positive control

    • WB: HeLa, HepG2, Jurkat and NIH3T3 nuclear lysates. IHC-P: Human thymus tissue.
  • General notes


    This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

Properties

Applications

Our Abpromise guarantee covers the use of ab5300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Can be blocked with Human NIPP1 peptide (ab23096).

Target

  • Function

    Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
    Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.
  • Sequence similarities

    Contains 1 FHA domain.
  • Domain

    Has a basic N- and C-terminal and an acidic central domain.
  • Post-translational
    modifications

    May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.
  • Cellular localization

    Nucleus. Nucleus speckle. Primarily, but not exclusively, nuclear and Cytoplasm. Found mainly in the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator of RNA decay antibody
    • ARD 1 antibody
    • ARD-1 antibody
    • ARD1 antibody
    • Homolog of E.coli RNase E antibody
    • NIPP 1 antibody
    • NIPP-1 antibody
    • NIPP1 antibody
    • Nuclear inhibitor of protein phosphatase 1 antibody
    • nuclear inhibitor of protein phosphatase-1 alpha antibody
    • Nuclear subunit of PP1 antibody
    • PP1R8_HUMAN antibody
    • PPP1R8 antibody
    • PRO2047 antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 8 antibody
    • Protein phosphatase 1 regulatory subunit 8 antibody
    • RNase E antibody
    • RNase E, E. coli, homolog of antibody
    see all

Images

  • All lanes : Anti-NIPP1 antibody (ab5300) at 1 µg/ml

    Lane 1 : HeLa nuclear lysate in RIPA buffer
    Lane 2 : HepG2 nuclear lysate in RIPA buffer
    Lane 3 : Jurkat nuclear lysate in RIPA buffer
    Lane 4 : NIH3T3 nuclear lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 40 kDa



    Primary incubation for 1 hour. Detected using chemiluminescence.

  • ab5300 (3.8µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

This product has been referenced in:

  • Nuytten M  et al. The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing. Oncogene 27:1449-60 (2008). WB . Read more (PubMed: 17724462) »
See 1 Publication for this product

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