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Cell Biology Apoptosis Mitochondrial
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NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab112149)

  • Datasheet
  • SDS
  • Protocol Booklet
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112149 NIR Mitochondrial Membrane Potential in Jurkat
  • 112149 NIR Mitochondrial Membrane Potential in HL60

Key features and details

  • Assay type: Direct
  • Platform: Flow cytometer
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry)
    See all Mitochondrial Membrane Potential kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Direct
  • Product overview

    NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) ab112149 is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential. The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.


    ab112149 uses our proprietary cationic NIR probe for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. In normal cells, the red fluorescence intensity is increased when the MitoNIR Dye is accumulated in the mitochondria. However, in apoptotic cells, the NIR stain intensity is decreased following the collapse of MMP. Cells stained with NIR Dye can be visualized with a flow cytometer at red excitation and far red emission (FL4 channel).


    ab112149 provides all the essential components. ab112149 can be used together with other reagents, such as blue laser excited propidium iodide and for studying cell vitality and apoptosis. ab112149 is optimized for screening apoptosis activators and inhibitors with a flow cytometer.

  • Notes

    Related assays

    Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay. 

    Review the metabolism assay guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • Platform

    Flow cytometer

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Assay Buffer 1 x 100ml
    MitoNIR Dye 1 x 500µl
  • Research areas

    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Kits/ Lysates/ Other
    • Kits
    • Apoptosis Kits
    • Other Apoptosis Kits
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Cell Viability and Senescence Kits
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Cell viability, plasma membrane damage
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Cell Damage
    • Kits/ Lysates/ Other
    • Kits
    • Apoptosis Kits
    • Transmembrane potential
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Membrane potential
  • Relevance

    Mitochondrial Membrane Potential is an important parameter of mitochondrial function used as an indicator of cell death. The collapse of the mitochondrial Membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
  • Alternative names

    • mitochondrial membrane potential

Associated products

  • Related Products

    • JC-1, Mitochondrial membrane potential dye (ab141387)

Images

  • 112149 NIR Mitochondrial Membrane Potential in Jurkat
    112149 NIR Mitochondrial Membrane Potential in Jurkat

    NIR Mitochondrial Membrane Potential in HL60 (ab112149). The decrease in fluorescence intensity of MitoNIR Dye with the addition of CCCP (ab141229) in Jurkat cells. Cells were loaded with MitoNIR Dye1x in the presence of DMSO alone (red),1µM CCCP (orange), 10µM CCCP(blue) and 50µM CCCP(green).

  • 112149 NIR Mitochondrial Membrane Potential in HL60
    112149 NIR Mitochondrial Membrane Potential in HL60

    NIR Mitochondrial Membrane Potential in HL60 (ab112149).  The decrease in fluorescence intensity of MitoNIR Dye with the addition of CCCP (ab141229) in HL60 cells. Cells were loaded with MitoNIR Dye1x in the presence of DMSO alone (red), 1µM CCCP (blue), 10µM CCCP (orange) and 50µM CCCP (green).

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab112149? Please let us know so that we can cite the reference in this datasheet.

ab112149 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Dear Sirs, May I have the excitation and emission spectra of NIR? I would like to combine with other fluorochromes, so this information is essential for us. Thanks a lot. Best regards

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Abcam community

Verified customer

Asked on Oct 02 2012

Answer

Thank you for contacting us.

The cationic NIR probe can be measured with a Flow cytometer in the FL4 channel (Ex/Em = 635/660 nm).

I hope this helps. Please do not hesitate to contact us for further assistance.

Read More

Abcam Scientific Support

Answered on Oct 02 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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